계명대학교 의학도서관 Repository

CYANATE AS A HEMOLYTIC FACTOR

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Affiliated Author(s)
문교철곽춘식김현철
Alternative Author(s)
Mun, Kyo CheolKwak, Chun SikKim, Hyun Chul
Journal Title
Renal Failure
ISSN
0886-022X
Issued Date
2000
Abstract
During advanced renal failure, and particularly in patients with end-stage renal disease, proteins are carbamylated as a result of a reaction with cyanate. If the carbamylation of proteins adversely alters their biologic activities and structures, then urea must be viewed as an uremic toxin, rather than a surrogate. Therefore, we studied in this paper the role of cyanate as a hemolytic factor of erythrocytes to explain anemia observed in patients with high blood urea levels due to inadequate dialysis. Cyanate was added to make the final concentration 150, 300 and 600 nmol to each test tube containing the final concentration of 140 x 10(6) with human erythrocytes per mL of phosphate buffered saline solution. And they were incubated at 37 degrees C for 24, 48 and 72 hours. The extent of hemolysis and carbamylation was monitored. The levels of hemolysis and carbamylated erythrocytes increased as the time of exposure to cyanate increased from 24 hours to 72 hours. Furthermore, those increased as cyanate concentration in the incubation media rose from 150 nmol to 600 nmol. Cyanate can induce hemolysis by carbamylation of erythrocytes. Urea, through cyanate, may contribute to hemolysis. If one extrapolates these results to patients with end-stage renal disease, it may help explain one of the reasons for the anemia in patients with high levels of BUN due to inadequate dialysis..
Department
Dept. of Biochemistry (생화학)
Dept. of Internal Medicine (내과학)
Publisher
School of Medicine
Citation
Kyo-Cheol Mun et al. (2000). CYANATE AS A HEMOLYTIC FACTOR. Renal Failure, 22(6), 809–814. doi: 10.1081/JDI-100101966
Type
Article
ISSN
0886-022X
DOI
10.1081/JDI-100101966
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34333
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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