Ultrastructural Evaluation of the Protective Effect of Nitroglycerin in Preservation-Reperfusion Injury of Rat Lungs
- K.Y. Kwon; C.H. Cho; Y.N. Kang; S.P. Kim; K.K. Park; D.Y. Keum; C.K. Park; S.H. Jheon
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- Aim of Study. Nitric oxide (NO) has been reported as a favorable protective supplement
in donor lung preservation, but related ultrastructural studies are rare in the literature.
This study was performed to assess the ultrastructural changes and to evaluate the
protective effect of NO as donor nitroglycerin (NTG) treatment of ischemia-reperfusion
injury in rat lungs.
Materials and Methods. Fifteen Sprague-Dawley rats weighing 300 to 350 g were used in
this study. The NTG group (n 5) used intravenous administration followed by mixture in the
University ofWisconsin (UW) solution. For the non-NTG group (n 5), we injected the same
amount of normal saline intravenously followed by admixture in the UW solution. The
heart-lung blocks were removed, weighed, and kept in UW solution for 24 hours at 10°C.
Reperfusion using human blood diluted in Krebs-Hensleit solution was done for 60 minutes.
For the control group (n 5), we injected the same amount of normal saline intravenously,
and removed the lungs with no preservation and reperfusion procedures.
Results. The non-NTG group showed multiple patchy areas of alveolar collapse with
marked swelling and destruction of type I epithelial cells, loss of type II cell surfactant
granules, endothelial swelling and papillary projection, interstitial edema, and alveolar
macrophages with active phagocytosis of the destroyed materials. The NTG group showed
similar ultrastructural changes, but in a lesser severity compared with the non-NTG group.
Conclusion. Administration of the NTG reduced the ischemia-reperfusion injury in the
rat donor lungs. Ultrastructural examination was an effective tool to evaluate the
protective effect of NTG in ischemia-reperfusion procedures of donor lungs.
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