Effect of Artificial Cells on Hepatic Function After Ischemia–Reperfusion Injury in Liver

E.J. ChangS.H. LeeK.C. MunS.I. SuhJ.H. BaeS.P. KimH.J. ChoiK.B. ChoJ.S. Hwang
Dept. of Physiology (생리학); Dept. of Pathology (병리학); Dept. of Microbiology (미생물학); Dept. of Internal Medicine (내과학); Dept. of Biochemistry (생화학); Institute for Medical Science (의과학연구소)
Issue Date
Transplantation Proceedings, Vol.36(7) : 1959-1961, 2004
Background: The liver suffers from ischemia/reperfusion injury during transplantation. Reactive oxygen species generated by xanthine oxidase during reperfusion of the ischemic liver may be partially responsible for the hepatic injury. Oxygen free radicals are removed by antioxidant enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase. Using glutaraldehyde and lysine we constructed crosslinked hemoglobin, containing SOD and catalase, and assessed its ability to protect against ischemia/ reperfusion injury during transplantation. Methods: In contrast to the sham-operated control groups, blood was exchanged using crosslinked hemoglobin (polyHb) a PolyHb–SOD–catalase (PSC) group. After ischemia/ reperfusion injury, several parameters of hepatic damage and oxygen free radicals were measured as well as microscopic examination. Results: Alanine aminotransferase, aspartate aminotransferase, superoxide produc- tion, hydrogen peroxide, and malondialdehyde levels were higher among the PolyHb group than sham-operated controls. The PolyHb group revealed a few apoptotic bodies, some acute inflammatory infiltrates in the sinusoids, nuclear fragmentations, cell shrinkage, and chromatin clumping with formation of apoptotic bodies in the apoptotic cells under microscopic examination. Alanine aminotransferase, aspartate aminotransferase, superoxide production, and hydrogen peroxide levels were lower in the PSC than the PolyHb group. Hepatic structures were well preserved in the PSC group. Conclusions: Reactive oxygen species contribute to hepatic dysfunction with morpho- logic changes. PSC is effective to reduce hepatic damage by lowering oxygen free radical–mediated injury after ischemia/reperfusion in the liver.
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
배재훈; 김상표; 서성일; 조광범; 황재석; 문교철
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