Apoptosis in Endothelial Cells by Cyclosporine
- E.A. Hwang; H.S. Kim; E. Ha; K.C. Mun
- Publication Year
- Objectives. The immunosuppressive drug cyclosporine (CsA) is a potent agent widely
used after organ transplantations and to treat various autoimmune disorders. After using
CsA, some patients suffer severe complications including renal and vascular toxicity, which
are inﬂuenced by the degree of the endothelial damage. Several studies have demonstrated
CsA treatment to directly induce apoptosis in several cell types. Thus, CsA may induce
endothelial damage via activation of proapoptotic proteins. The present study was
undertaken to investigate the effects of CsA on apoptosis of endothelial cells using human
umbilical vein endothelial cells.
Methods. Proliferation was measured by using the Cell Counting Assay Kit after cells
were exposed to CsA (0 L, 10 L, 30 L, 50 L or 100 g/mL). Apoptotic cells were identiﬁed
by ﬂuorescence microscopy of 4=, 6-diamidino-2-phenylidole-stained nuclei. Western blot
analysis was done for poly(ADP-ribose) polymerase (PARP), p27, p53 and caspase.
Results. Cell viability decreased dependent on the CsA concentration. CsA treatment
group showed chromatin condensation and nuclear fragmentation. CsA produced a
dose-dependent induction of p27 and reduction of procasapase-3. CsA treatment induced
the degradation of 116-kDa PARP into an 89-kDa fragment.
Conclusions. CsA induced apoptosis of endothelial cells.
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