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E2F Decoy Oligodeoxynucleotides on Neointimal Hyperplasia in Canine Vein Graft

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Author(s)
조원현김형태이인규
Alternative Author(s)
Cho, Won HyunKim, Hyoung TaeLee, In Kyu
Publication Year
2005
Abstract
Double-stranded DNA with high affinity to E2F as a decoy cis-element blocks the
activation of genes mediating the cell cycle, resulting in effective suppression of the
smooth muscle cell proliferation that causes intimal hyperplasia. To evaluate the effect
of the E2F decoy to suppress neointimal hyperplasia autogenous venous bypass grafts
were performed in dogs after incubation with heparin (group 1), with E2F decoy
oligodeoxynucleotides (ODN) (groups 2 and 3), or with a random ODN (group 4)
using a Japan-liposomeal method based on a hemagglutinating virus. The intimal and
medial cross-sectional surface area of the anastomotic site was measured at 4 months
after bypass surgery in groups 1, 3, and 4 by computerized planimetry and at 4 weeks
in group 2 to compare the intimal/medial (I/M) area ratios. Autogenous vein grafts
treated with E2F decoy showed a significant reduction in I/M area ratio (0.26± 0.11)
compared with the heparin-treated control group (1.49 ± 0.29) or the mismatched
ODN-treated group (1.61 ± 0.28; P = .000). There was no difference in the I/M area
ratio according to experimental periods (groups 2 vs 3: 0.26 ± 0.11 vs 0.37 ±
0.32; P =.446) or the anastomotic sites (proximal vs distal; P = .934). In conclusion,
an E2F decoy can suppress neointimal hyperplasia in autogenous vein grafts, which
may prolong patency by reducing graft stenosis.
Department
Dept. of Surgery (외과학)
Dept. of Internal Medicine (내과학)
Institute for Medical Science (의과학연구소)
Publisher
School of Medicine
Citation
Transplantation Proceedings, Vol.37(1) : 77-79, 2005
Type
Article
ISSN
0041-1345
DOI
10.1016/j.transproceed.2004.11.021
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34704
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