Effect of AP-1 Decoy Using Hemagglutinating Virus of Japan-Liposome on the Intimal Hyperplasia of the Autogenous Vein Graft in Mongrel Dogs
- 조원현; 김형태
- Alternative Author(s)
- Cho, Won Hyun; Kim, Hyoung Tae
- Publication Year
- Intimal hyperplasia is the leading cause of late vein graft failure. Smooth muscle cell
proliferation and migration is the underlying mechanism. Pharmacological approaches to
prolong vein graft patency have produced limited results. AP-1 proteins play a role in the
expression of many genes involved in cellular proliferation and cell cycle progression.
Previously we reported inhibition of vascular smooth muscle cell migration, proliferation,
and intimal hyperplasia in the balloon-injured rat carotid artery using an AP-1 decoy with
HVJ-liposomes. In this report, we evaluated the effect of the AP-1 decoy on intimal
hyperplasia in a large animal model. The jugular vein was transfected with hemaggluti-
nating virus of Japan-liposomes containing the AP-1 decoy or scrambled oligonucleotides.
An interposition graft was performed with the pretreated jugular vein between the
transected femoral arteries. The graft was harvested at 16 weeks after the procedure. The
intimal area was compared: the intimal area of the AP-1 decoy-treated versus control
group was 47.3±15.2 versus 102.3± 15.9 (P < .05), respectively. In conclusion, AP-1
decoy using HVJ-liposomes effectively prevented intimal hyperplasia of an autogenous
vein graft in mongrel dogs.
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