Effects of Cyclosporine on the Antioxidant Status and Oxidative Stress in the Glioma Cells
- K.H. Mun; E.Y. Ha
- Publication Year
- Objectives. After organ transplantation, some patients suffer neurological complica-
tions. Among the immunosuppressants, cyclosporine can cause neurological side effects.
However, the mechanisms of encephalopathy by cyclosporine are not fully understood.We
measured the antioxidant status, the hydrogen peroxide level, and the malondialdehyde
level in glioma cells after cyclosporine treatment.
Methods. The production of hydrogen peroxide was determined using a modiﬁed
xylenol orange method. The amount of malondialdehyde was measured using the
thiobarbituric acid assay. Total antioxidant status was measured using Free Radical
Analytical System 4 with kits.
Results. Cyclosporine resulted in the production of hydrogen peroxide by the glioma
cells. The increased production of hydrogen peroxide depended on the drug concen-
tration. The antioxidant status was decreased in the glioma cells after cyclosporine
treatment. The malondialdehyde level was not changed in glioma cells after cyclospor-
Conclusions. Increased production of reactive oxygen species and decreased antioxdant
status by cyclosporine in glioma cells may contribute to neurological side effects in
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