Effects of Epigallocatechin Gallate on the Hemolysis Induced by Cyclosporine

Authors
H.C. KimE.J. ChangK.C. Mun
Department
Dept. of Biochemistry (생화학); Dept. of Internal Medicine (내과학); Kidney Institute (신장연구소); Institute for Medical Science (의과학연구소)
Issue Date
2005
Citation
Transplantation Proceedings, Vol.37(5) : 2385-2386, 2005
ISSN
0041-1345
Abstract
Introduction Hemolysis is one of the side effects of cyclosporine (CsA) therapy, in part due to, increased production of free radical species by CsA. Epigallocatechin gallate (EGCG), which acts as a highly efficient free radical scavenger, may have a protective effect on CsA-induced hemolysis. In this study, we measured the degree of hemolysis of as well as the amounts of hydrogen peroxide level and malondialdehyde produced by normal human erythrocytes (RBCs) incubated with CsA and with EGCG. Methods Human RBCs were incubated as follows. In group 1, 4.2 × 106/mL RBCs were incubated with Cremophore EL. In group 2, the RBCs were incubated with only 167 μg/mL EGCG. In group 3, the RBCs were incubated with 1.67 mg/mL CsA. In group 4, the RBCs were incubated with CsA plus EGCG. Results The degree of hemolysis in group 2 (53.8 ± 3.8) was significantly higher than that in group 1 (7.0 ± 1.0). The degree of hemolysis in group 3 (86.2 ± 2.2) was significantly higher than that in group 1 and group 2. The degree of hemolysis in group 4 (74.9 ± 2.9) was significantly higher than in group 1 and in group 2, but lower than that in group 3. The hydrogen peroxide and malondialdehyde levels paralleled the degree of hemolysis. Conclusions These results suggest that CsA can induce free radical-mediated hemolysis, which can be partially prevented with EGCG.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34744
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Kidney Institute (신장연구소)
Keimyung Author(s)
문교철; 김현철
Full Text
https://www.sciencedirect.com/science/article/pii/S0041134505002411?via%3Dihub
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