Effects of Tacrolimus on Antioxidant Status and Oxidative Stress in Glioma Cells
- K.B. Jin; E.A. Hwang; S.Y. Han; S.B. Park; H.C. Kim; E.Y. Ha; S.I. Suh; K.C. Mun
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- Objectives. After organ transplantation, some patients suf fer from mild neurologic
symptoms, ranging from tremor to severe complications, including seizures and encepha-
lopathy. Among the immunosuppressants, tacrolimus can cause neurologic side ef fects.
However, the mechanisms of encephalopathy by tacrolimus are not fully understood. We
measured the antioxidant status, hydrogen peroxide level, and malondialdehyde level in
glioma cells after tacrolimus treatment.
Methods. The production of hydrogen peroxide was determined by the modiﬁed xylenol
orange method. The amount of malondialdehyde was measured by the thiobarbituric acid
assay, which is based on malondialdehyde reaction with thiobarbituric acid to give a red
species absorbing at 535 nm. Total antioxidant status (TAS) was measured using TAS kits
Results. Tacrolimus resulted in dose- and time-dependent increases in the production of
hydrogen peroxide by glioma cells. The antioxidant status decreased in the glioma cells
after tacrolimus treatment. Malondialdehyde level was unchanged in the glioma cells after
Conclusions. Increased production of reactive oxygen species and decreased antioxidant
status by tacrolimus in glioma cells may contribute to neurologic side ef fects.
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