Effects of Tacrolimus on Antioxidant Status and Oxidative Stress in Glioma Cells

Authors
K.B. JinE.A. HwangS.Y. HanS.B. ParkH.C. KimE.Y. HaS.I. SuhK.C. Mun
Department
Dept. of Biochemistry (생화학); Dept. of Internal Medicine (내과학); Dept. of Microbiology (미생물학); Kidney Institute (신장연구소)
Issue Date
2008
Citation
Transplantation Proceedings, Vol.40(8) : 2740-2741, 2008
ISSN
0041-1345
Abstract
Objectives. After organ transplantation, some patients suf fer from mild neurologic symptoms, ranging from tremor to severe complications, including seizures and encepha- lopathy. Among the immunosuppressants, tacrolimus can cause neurologic side ef fects. However, the mechanisms of encephalopathy by tacrolimus are not fully understood. We measured the antioxidant status, hydrogen peroxide level, and malondialdehyde level in glioma cells after tacrolimus treatment. Methods. The production of hydrogen peroxide was determined by the modified xylenol orange method. The amount of malondialdehyde was measured by the thiobarbituric acid assay, which is based on malondialdehyde reaction with thiobarbituric acid to give a red species absorbing at 535 nm. Total antioxidant status (TAS) was measured using TAS kits (NX2332). Results. Tacrolimus resulted in dose- and time-dependent increases in the production of hydrogen peroxide by glioma cells. The antioxidant status decreased in the glioma cells after tacrolimus treatment. Malondialdehyde level was unchanged in the glioma cells after tacrolimus treatment. Conclusions. Increased production of reactive oxygen species and decreased antioxidant status by tacrolimus in glioma cells may contribute to neurologic side ef fects.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34745
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Kidney Institute (신장연구소)
Keimyung Author(s)
하은영; 문교철; 진규복; 황은아; 한승엽; 박성배; 김현철; 서성일
Full Text
https://www.sciencedirect.com/science/article/pii/S0041134508010567?via%3Dihub
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