The Production of Reactive Oxygen Species in Tacrolimus-Treated Glial Cells

Authors
K.B. JinH.J. ChoiH.T. KimE.A. HwangS.I. SuhS.Y. HanS.I. NamS.B. ParkH.C. KimE.Y. HaK.C. Mun
Department
Dept. of Internal Medicine (내과학); Dept. of Biochemistry (생화학); Dept. of Otorhinolaryngology (이비인후과학); Dept. of Microbiology (미생물학); Dept. of Surgery (외과학); Kidney Institute (신장연구소)
Issue Date
2008
Citation
Transplantation Proceedings, Vol.40(8) : 2680-2681, 2008
ISSN
0041-1345
Abstract
Objective After organ transplantation, some patients suffer from mild neurological symptoms, such as tremor, to severe complications, including seizures and encephalopathy. These neurological side effects can be caused by immunosuppressants such as tacrolimus. However, the mechanism of encephalopathy by tacrolimus is not fully understood. Methods We measured the production of reactive oxygen species (ROS) in glioma cells after tacrolimus treatment. Tacrolimus added to glioma cells was incubated for 60 minutes at 37°C. The production of ROS was evaluated by measuring the fluorescent product from the oxidation of an oxidant-sensitive 2′,7′-dichlorofluorescin using VICTOR3TM multilabel counter. Results Tacrolimus resulted in the production of the ROS in glioma cells. The production of the ROS was increased in time-dependent fashion. Conclusions These findings indicated that the tacrolimus may contribute the neurological side effects by ROS production.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34768
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Kidney Institute (신장연구소)
Keimyung Author(s)
진규복; 황은아; 한승엽; 박성배; 김현철; 하은영; 문교철; 남성일; 서성일; 김형태
Full Text
https://www.sciencedirect.com/science/article/pii/S0041134508011111?via%3Dihub
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