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The Production of Reactive Oxygen Species in Tacrolimus-Treated Glial Cells

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Author(s)
진규복황은아한승엽박성배김현철하은영문교철남성일서성일김형태
Alternative Author(s)
Jin, Kyu BokHwang, Eun AhHan, Seung YeupPark, Sung BaeKim, Hyun ChulHa, Eun YoungMun, Kyo CheolNam, Sung IlSuh, Seong IlKim, Hyoung Tae
Publication Year
2008
Abstract
Objective

After organ transplantation, some patients suffer from mild neurological symptoms, such as tremor, to severe complications, including seizures and encephalopathy. These neurological side effects can be caused by immunosuppressants such as tacrolimus. However, the mechanism of encephalopathy by tacrolimus is not fully understood.

Methods

We measured the production of reactive oxygen species (ROS) in glioma cells after tacrolimus treatment. Tacrolimus added to glioma cells was incubated for 60 minutes at 37°C. The production of ROS was evaluated by measuring the fluorescent product from the oxidation of an oxidant-sensitive 2′,7′-dichlorofluorescin using VICTOR3TM multilabel counter.

Results

Tacrolimus resulted in the production of the ROS in glioma cells. The production of the ROS was increased in time-dependent fashion.

Conclusions

These findings indicated that the tacrolimus may contribute the neurological side effects by ROS production.
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