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Frequent Loss of Imprinting of the H19 and IGF-II Genes in Ovarian Tumors

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Author(s)
이태성장성익
Alternative Author(s)
Lee, Tae SungChang, Sung Ik
Publication Year
1998
Abstract
Several human imprinted genes have been
identified and are implicated in genetic dis-
eases and tumorigenesis. We studied alter-
ations of two imprinted genes, the pater-
nally imprinted H19 and maternally im-
printed IGF2, in 15 ovarian tumors with
various cell types. To know allele-specific
expression of the two genes, we analyzed re-
striction fragment length polymorphisms
(RFLPs) at the 3*-untranslated region (UTR)
in their cDNA, compared with those in the
respective genomic DNA. As a result, bialle-
lic H19 and IGF2 expression was observed in
8 (62%) of 13 informative (heterozygous)
ovarian cancers and in 6 of 11 informative
cases, respectively. H19 loss of imprinting
(LOI) was most frequently observed in ma-
lignant serous cystadenocarcinoma (in four
of six cases), whereas IGF2 LOI was not com-
mon in malignant epithelial cancers be-
cause three of six such LOI events occurred
in benign mucinous cystadenomas and non-
cancerous endometriotic cyst. Our data sug-
gest that the alteration of H19 and IGF2 im-
printing plays differential roles in tumori-
genesis and progression of ovarian tumors,
depending on the tissue type as well as the
developmental stage. Our data may argue
against tumor suppressor activity of H19 in
ovarian cancers. Am. J. Med. Genet. 80:391–
395, 1998.
© 1998 Wiley-Liss, Inc.
KEY WORDS: genomic imprinting; H19;
IGF2; ovarian cancer; carci-
nogenesis
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Dept. of Anatomy (해부학)
Publisher
School of Medicine
Citation
American Journal of Medical Genetics, Vol.80(4) : 391-395, 1998
Type
Article
ISSN
0148-7299
DOI
10.1002/(SICI)1096-8628(19981204)80:4<391::AID-AJMG16>3.0.CO;2-H
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34929
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