Effect of PGC-1α on TNF-α-Induced MCP-1 and VCAM-1 Expression and NF-κB Activation in Human Aortic Smooth Muscle and Endothelial Cells

Authors
Hye-Jin KimKeun-Gyu ParkEun-Kyung YooYoung-Ho KimYoon-Nyun KimHye-Soon KimHyoung Tae KimJoong-Yeol ParkKi-Up LeeWon Gu JangJung-Guk KimBo-Wan KimIn-Kyu Lee
Department
Dept. of Internal Medicine (내과학)
Issue Date
2007
Citation
Antioxidants & Redox Signaling, Vol.9(3) : 301-307, 2007
ISSN
1523-0864
Abstract
Increased oxidative stress in vascular cells is implicated in the pathogenesis of atherosclerosis. Reactive oxygen species (ROS) induce vascular inflammation via the proinflammatory cytokine/NF-κB pathway. Several lines of evidence suggest that peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC-1α) is an important regulator of intracellular ROS levels. However, no studies have examined the effects of PGC-1α on this process. We investigated the effects of PGC-1α on inflammatory molecule expression and activity of the redox-sensitive transcription factor, NF-κB, in vascular cells. PGC-1α expressed in human aortic smooth (HASMCs) and endothelial cells (HAECs) is upregulated by AMP-activated protein kinase activators, including metformin, rosiglitazone and α-lipoic acid. Tumor necrosis factor-α (TNF-α), a major proinflammatory factor in the development of vascular inflammation, stimulates intracellular ROS production through an increase in both mitochondrial ROS and NAD(P)H oxidase activity. Adenovirus-mediated overexpression of the PGC-1α gene in HASMCs and HAECs leads to a significant reduction in intracellular and mitochondrial ROS production as well as NAD(P)H oxidase activity. Consequently, NF-κB activity and MCP-1 and VCAM- 1 induced by TNF-α are suppressed. Our data support the possibility that agents stimulating PGC-1α expression in the vasculature aid in preventing the development of atherosclerosis.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35065
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
Keimyung Author(s)
김윤년; 김혜순; 이인규
Full Text
https://www.liebertpub.com/doi/abs/10.1089/ars.2006.1456
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