Induction of apoptosis by withaferin A in human leukemia U937 cells through down-regulation of Akt phosphorylation

Authors
Jung Hwa OhTae-Jin LeeSang Hyun KimYung Hyun ChoiSang Han LeeJin Man LeeYoung-Ho KimJong-Wook ParkTaeg Kyu Kwon
Department
Dept. of Immunology (면역학)
Issue Date
2008
Citation
Apoptosis, Vol.13(12) : 1494-1504, 2008
ISSN
1360-8185
Abstract
Withaferin A, a major chemical constituent of Withania somnifera, has been reported for its tumor cell growth inhibitory activity, antitumor effects, and impairing metastasis and angiogenesis. The mechanism by which withaferin A initiates apoptosis remains poorly understood. In the present report, we investigated the effect of withaferin A on the apoptotic pathway in U937 human promonocytic cells. We show that withaferin A induces apoptosis in association with the activation of caspase-3. JNK and Akt signal pathways play crucial roles in withaferin A-induced apoptosis in U937 cells. Furthermore, we have shown that overexpression of Bcl-2 and active Akt (myr-Akt) in U937 cells inhibited the induction of apoptosis, activation of caspase-3, and PLC-c1 cleavage by withaferin A. Taken together, our results indicated that the JNK and Akt pathways and inhibition of NF-jB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells. Keywords Withaferin A Apoptosis pAkt U937 JNK XIAP
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35071
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
Keimyung Author(s)
박종욱; 권택규
Full Text
https://link.springer.com/article/10.1007%2Fs10495-008-0273-y
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