NAG-1 up-regulation mediated by EGR-1 and p53 is critical for quercetin-induced apoptosis in HCT116 colon carcinoma cells

Authors
J. H. LimJ.-W. ParkD. S. MinJ.-S. ChangY. H. LeeY. B. ParkK. S. ChoiT. K. Kwon
Department
Dept. of Immunology (면역학)
Issue Date
2007
Citation
Apoptosis, Vol.12(2) : 411-421, 2007
ISSN
1360-8185
Abstract
Quercetin, a flavonoid molecule ubiquitously present in nature, has multiple effects on cancer cells, including the inhibition of cell proliferation and migration. However, the responsible molecular mechanisms are not fully understood. We found that quercetin induces the expression of NAG-1 (Non-steroidal anti-inflammatory drug activated gene-1), a TGF-β superfamily protein, during quercetininduced apoptosis of HCT116 human colon carcinoma cells. Reporter assays using the luciferase constructs contain-ing NAG-1 promoter region demonstrate that early growth response-1 (EGR-1) and p53 are required for quercetinmediated activation of the NAG-1 promoter. Overexpression ofNAG-1 enhanced the apoptotic effect of quercetin, but suppression of quercetin-induced NAG-1 expression by NAG-1 siRNA attenuated quercetin-induced apoptosis in HCT116 cells. Taken together, the present study demonstrates for the first time that quercetin induces apoptosis via NAG-1, providing a mechanistic basis for the apoptotic effect of quercetin in colon carcinoma cells. Keywords Quercetin . NAG-1 . Apoptosis . Sp1 . EGR-1 . p53 . Colon carcinoma cells
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35072
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
Keimyung Author(s)
박종욱; 권택규
Full Text
https://link.springer.com/article/10.1007%2Fs10495-006-0576-9
File in this Item
 사서에게 요청하기
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE