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Flavonoids Inhibit Histamine Release and Expression of Proinflamma-tory Cytokines in Mast Cells

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Author(s)
Hyo-Hyun ParkSoyoung LeeHee-Young SonSeung-Bin ParkMi-Sun KimEun-Ju ChoiThoudam S.K. SinghJeoung-Hee HaMaan-Gee LeeJung-Eun KimMyung Chul HyunTaeg Kyu KwonYeo Hyang KimSang-Hyun Kim
Publication Year
2008
Abstract
Mast cells participate in allergy and inflammation by secreting inflammatory mediators such as histamine
and proinflammatory cytokines. Flavonoids are naturally occurring molecules with antioxidant,
cytoprotective, and antiinflammatory actions. However, effect of flavonoids on the release of histamine
and proinflammatory mediator, and their comparative mechanism of action in mast cells were not well
defined. Here, we compared the effect of six flavonoids (astragalin, fisetin, kaempferol, myricetin, quercetin,
and rutin) on the mast cell-mediated allergic inflammation. Fisetin, kaempferol, myricetin, quercetin,
and rutin inhibited IgE or phorbol-12-myristate 13-acetate and calcium ionophore A23187
(PMACI)-mediated histamine release in RBL-2H3 cells. These five flavonoids also inhibited elevation
of intracellular calcium. Gene expressions and secretion of proinflammatory cytokines such as tumor
necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 were assessed in PMACI-stimulated
human mast cells (HMC-1). Fisetin, quercetin, and rutin decreased gene expression and production of
all the proinflammatory cytokines after PMACI stimulation. Myricetin attenuated TNF-α and IL-6 but
not IL-1β and IL-8. Fisetin, myricetin, and rutin suppressed activation of NF-κB indicated by inhibition
of nuclear translocation of NF-κB, NF-κB/DNA binding, and NF-κB-dependent gene reporter assay.
The pharmacological actions of these flavonoids suggest their potential activity for treatment of allergic
inflammatory diseases through the down-regulation of mast cell activation.
Department
Dept. of Immunology (면역학)
Dept. of Pediatrics (소아청소년학)
Publisher
School of Medicine
Citation
Archives of Pharmacal Research, Vol.31(10) : 1303-1311, 2008
Type
Article
ISSN
0253-6269
DOI
10.1007/s12272-001-2110-5
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35089
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