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E2F decoy oligodeoxynucleotides effectively inhibit growth of human tumor cells

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Author(s)
박근규이인규박관규
Alternative Author(s)
Park, Keun GyuLee, In KyuPark, Kwan Kyu
Publication Year
2003
Abstract
Abnormal cell proliferation, largely dependent upon deregulation of cell-cycle regulatory proteins, is an important feature of several forms of human cancer. The transcription factor, E2F, plays a critical role in the trans-activation of several genes involved in cell-cycle regulation, thereby regulating cell growth. We have demonstrated that E2F decoy oligodeoxynucleotides (ODNs) with a circular dumbbell structure (CD-E2F decoy) corresponding to E2F binding sites effectively inhibit cell proliferation of primary cultured cells. Here we found that the E2F decoy ODNs inhibited serum-induced promoter activity of E2F-dependent genes in a sequence-specific manner in a RB-positive human osteosarcoma, U2OS, as well as in a RB-negative human cervical carcinoma, C33A. This E2F decoy ODN strongly inhibited gene expression of endogenous E2F1 and PCNA and proliferation of these cancer cells. Our results suggest that this decoy ODN strategy could represent a powerful investigative and potentially therapeutic strategy in the prevention and treatment of cancer.

Keywords
E2F;
RB;
Decoy;
Dumbbell;
Tumor growth inhibition;
Osteosarcoma;
Cervical carcinoma
Department
Dept. of Internal Medicine (내과학)
Dept. of Pathology (병리학)
Kidney Institute (신장연구소)
Publisher
School of Medicine
Citation
Biochemical and Biophysical Research Communications, Vol.310(4) : 1048-1053, 2003
Type
Article
ISSN
0006-291X
DOI
10.1016/j.bbrc.2003.09.124
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35159
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