Induction of cyclooxygenase-2 in macrophages by catalase: role of NF-κB and PI3K signaling pathways

Authors
Byeong-Churl JangDo-Hyun KimJong-Wook ParkTaeg Kyu KwonSang-Pyo KimDae-Kyu SongJong-Gu ParkJae-Hoon BaeKyo-Chul MunWon-Ki BaekMin-Ho SuhTimothy HlaSeong-Il Suh
Department
Dept. of Molecular Medicine (분자의학); Dept. of Immunology (면역학); Dept. of Pathology (병리학); Dept. of Biochemistry (생화학); Dept. of Microbiology (미생물학); Dept. of Physiology (생리학); Institute for Medical Science (의과학연구소)
Issue Date
2004
Citation
Biochemical and Biophysical Research Communications, Vol.316(2) : 398-406, 2004
ISSN
0006-291X
Abstract
Induction of COX-2 by catalase in smooth muscle cells, endothelial cells, and neuronal cells has been previously reported. However, the mechanism by which catalase up-regulates COX-2 remains poorly understood. In this study, we investigated the effect of catalase on induction of COX-2 in macrophages. The addition of catalase into Raw 264.7 macrophages induced COX-2 expression that was correlated with increased COX-2 transcription and mRNA stability. Catalase also induced activation of NF-κB, PI3K, ERKs, p38s, or JNKs. Catalase-induced COX-2 expression was abrogated by treatment of MG-132 (a NF-κB inhibitor) or LY294002 (a PI3K inhibitor), but not by treatment of PD98059 (an ERK inhibitor), SB203580 (a p38 inhibitor), or SP600125 (a JNK inhibitor). Moreover, inhibition of PI3K by LY294002 caused partial decrease of catalase-induced COX-2 transcription and steady-state COX-2 transcript levels, but not COX-2 mRNA stability. Together, these results suggest that catalase induces the expression of COX-2 in Raw 264.7 macrophages, and the induction is related with activation of NF-κB transcription factor and PI3K signaling pathway. Keywords Catalase; Cyclooxygenase-2; NF-κB; PI3K; Raw 264.7 cells
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35163
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
장병철; 박종욱; 권택규; 김상표; 송대규; 박종구; 배재훈; 문교철; 백원기; 서민호; 서성일
Full Text
https://linkinghub.elsevier.com/retrieve/pii/S0006291X04003183
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