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Involvement of ERK and Protein Tyrosine Phosphatase Signaling Pathways in EGCG-Induced Cyclooxygenase-2 Expression in Raw 264.7 Cells

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Author(s)
박종욱권택규서성일
Alternative Author(s)
Park, Jong WookKwon, Taeg KyuSuh, Seong Il
Publication Year
2001
Abstract
Prostaglandins play regulatory roles in a variety of physiological and pathological processes in immune response and inflammation. Epigallocatechin-3-gallate (EGCG) is known to potent antitumor agent with antioxidant property. We first investigated the effect of EGCG on the production of prostaglandin E2 (PGE2) and the expression of cyclooxygenase-2 (COX-2), the rate-limiting enzyme in the synthesis of PGE2, using macrophage cell line, Raw264.7. Our results showed that COX-2 expression and PGE2 production are upregulated by EGCG treatment and that this induction of COX-2 is regulated in part at the transcriptional level. In addition, we demonstrated the signal transduction pathway of mitogen-activated protein kinase (MAP kinase) in EGCG-mediated COX-2 expression. The MEK inhibitor (PD098059) prevented EGCG-induced COX-2 expression, whereas sodium orthovanadate (protein-tyrosine phosphatase inhibitor) significantly enhanced COX-2 expression and PGE2 production. These results suggest that EGCG mediated COX-2 expression and PGE2 production is associated with the activation of both the ERK and protein-tyrosine phosphatase signaling pathways.

Keywords
EGCG;
COX-2;
signal transduction;
prostaglandin, COX-2 promoter
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
Biochemical and Biophysical Research Communications, Vol.286(4) : 721-725, 2001
Type
Article
ISSN
0006-291X
DOI
10.1006/bbrc.2001.5415
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35165
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