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Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-κB

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Author(s)
이인규
Alternative Author(s)
Lee, In Kyu
Publication Year
2003
Abstract
This study investigated the effect of oleic acid on the expression levels of endothelin-1 (ET-1) and on the signaling pathways mediating it in human aortic endothelial cells (HAECs). ET-1 mRNA expression was significantly increased by oleic acid in a dose- and time-dependent manner. Elevation of ET-1 expression in response to oleic acid was inhibited by the protein kinase C (PKC) inhibitor, GF109203X, or the NF-κB inhibitor, pyrrolidine dithiocarbamate. In addition, both PKC and NF-κB activities were significantly increased by oleic acid. Immunoblot analysis revealed that conventional PKCs (PKC-α and -βII isoforms) were significantly increased in the membranous fractions of HAECs treated with oleic acid. PKC inhibitor completely abolished oleic acid-induced NF-κB activation, suggesting that PKC activation is upstream of NF-κB activation in oleic acid-induced ET-1 expression. These data suggest that elevated plasma oleic acid levels observed in obese, insulin-resistant subjects result in endothelial dysfunction, at least in part, through an increase in ET-1 expression.

Keywords
Endothelin-1;
Oleic acid;
Protein kinase C;
Nuclear factor κB
Department
Dept. of Internal Medicine (내과학)
Publisher
School of Medicine
Citation
Biochemical and Biophysical Research Communications, Vol.303(3) : 891-895, 2003
Type
Article
ISSN
0006-291X
DOI
10.1016/S0006-291X(03)00436-4
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35167
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