Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-κB

Authors
Joong-Yeol ParkYun Mi KimHai Sun SongKi Young ParkYoung Mi KimMin Seon KimYoungmi Kim PakIn Kyu LeeJae Dam LeeSeung-Jung ParkKi-Up Lee
Department
Dept. of Internal Medicine (내과학)
Issue Date
2003
Citation
Biochemical and Biophysical Research Communications, Vol.303(3) : 891-895, 2003
ISSN
0006-291X
Abstract
This study investigated the effect of oleic acid on the expression levels of endothelin-1 (ET-1) and on the signaling pathways mediating it in human aortic endothelial cells (HAECs). ET-1 mRNA expression was significantly increased by oleic acid in a dose- and time-dependent manner. Elevation of ET-1 expression in response to oleic acid was inhibited by the protein kinase C (PKC) inhibitor, GF109203X, or the NF-κB inhibitor, pyrrolidine dithiocarbamate. In addition, both PKC and NF-κB activities were significantly increased by oleic acid. Immunoblot analysis revealed that conventional PKCs (PKC-α and -βII isoforms) were significantly increased in the membranous fractions of HAECs treated with oleic acid. PKC inhibitor completely abolished oleic acid-induced NF-κB activation, suggesting that PKC activation is upstream of NF-κB activation in oleic acid-induced ET-1 expression. These data suggest that elevated plasma oleic acid levels observed in obese, insulin-resistant subjects result in endothelial dysfunction, at least in part, through an increase in ET-1 expression. Keywords Endothelin-1; Oleic acid; Protein kinase C; Nuclear factor κB
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35167
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
Keimyung Author(s)
이인규
Full Text
https://linkinghub.elsevier.com/retrieve/pii/S0006291X03004364
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