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Glucosamine induces autophagic cell death through the stimulation of ER stress in human glioma cancer cells

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Author(s)
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Alternative Author(s)
Baek, Won Ki
Publication Year
2010
Abstract
Autophagy can promote cell survival or death, but the molecular basis of its dual role in cancer is not well understood. Here, we report that glucosamine induces autophagic cell death through the stimulation of endoplasmic reticulum (ER) stress in U87MG human glioma cancer cells. Treatment with glucosamine reduced cell viability and increased the expression of LC3 II and GFP-LC3 fluorescence puncta, which are indicative of autophagic cell death. The glucosamine-mediated suppression of cell viability was reversed by treatment with an autophagy inhibitor, 3-MA, and interfering RNA against Atg5. Glucosamine-induced ER stress was manifested by the induction of BiP, IRE1α, and phospho-eIF2α expression. Chemical chaperon 4-PBA reduced ER stress and thereby inhibited glucosamine-induced autophagic cell death. Taken together, our data suggest that glucosamine induces autophagic cell death by inducing ER stress in U87MG glioma cancer cells and provide new insight into the potential anticancer properties of glucosamine.
Department
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
Biochemical and Biophysical Research Communications, Vol.399(1) : 111-116, 2010
Type
Article
ISSN
0006-291X
DOI
10.1016/j.bbrc.2010.07.050
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35198
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