Silibinin inhibits expression of HIF-1α through suppression of protein translation in prostate cancer cells

Authors
Hui-Jung JungJong-Wook ParkJason S. LeeSeong-Ryong LeeByeong-Churl JangSeong-Il SuhMin-Ho SuhWon-Ki Baek
Department
Dept. of Immunology (면역학); Dept. of Pharmacology (약리학); Dept. of Molecular Medicine (분자의학); Dept. of Microbiology (미생물학)
Issue Date
2009
Citation
Biochemical and Biophysical Research Communications, Vol.390(1) : 71-76, 2009
ISSN
0006-291X
Abstract
Silibinin is a polyphenolic flavonoid isolated from the milk thistle (Silybum marianum) and is reported to exhibit anticancer properties. Recently, it has been reported that silibinin inhibits hypoxia-inducible factor-1α (HIF-1α) expression in cancer cells. However, the precise mechanism by which silibinin decreases HIF-1 expression is not fully understood. In this study, silibinin inhibited basal and hypoxia induced expression levels of HIF-1α protein in LNCaP and PC-3 prostate cancer cells, while the rate of HIF-1α protein degradation and mRNA levels were not affected. We found that the decrease in HIF-1 protein by silibinin correlated with suppression of de novo synthesis of HIF-1α protein. Silibinin inhibited global protein synthesis coincided with reduction of eIF4F complex formation and induction of phosphorylation of the translation initiation factor 2α (eIF-2α) which can cause inhibition of general protein synthesis. These results suggest that silibinin’s activity to inhibit HIF-1α protein expression is associated with the suppression of global protein translation. Keywords Hypoxia-inducible factor-1α; Silibinin; Protein translation; Prostate cancer
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35202
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
Keimyung Author(s)
박종욱; 이성용; 장병철; 서성일; 서민호; 백원기
Full Text
https://linkinghub.elsevier.com/retrieve/pii/S0006291X09018774
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