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Propofol attenuates Kupffer cell activation during hypoxia-reoxygenation [Le propofol diminue ľactivation des cellules de Kupffer pendant ľhypoxie-réoxygénation]

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Affiliated Author(s)
배재훈
Alternative Author(s)
Bae, Jae Hoon
Journal Title
Canadian Journal of Anesthesia
ISSN
0832-610X
Issued Date
2005
Abstract
PURPOSE: We undertook a study to determine whether propofol may attenuate Kupffer cell (KC) activation, thus protecting the cells against hypoxia-reoxygenation injury through the modulation of intracellular calcium ([Ca2+]i).

METHODS: [Ca2+]i, the expression of tumour necrosis factor (TNF)-alpha mRNA, and KC viability were measured in response to hypoxia-reoxygenation following pretreatment with propofol 0.5 and 5 microg.mL(-1) (Groups P1 and P2, respectively) or without propofol (Group HRC). KCs were isolated and cultured from male Sprague-Dawley rats. KCs were incubated under an atmosphere of hypoxia (95% N2 + 5% CO2) for 60 min with subsequent 120 min reoxygenation (95% air + 5% CO2). [Ca2+]i for the first 12 min after reoxygenation, TNF-alpha mRNA, and KC viability at the end of reoxygenation in groups P1 and P2 were compared with those of HRC.

RESULTS: The increase of [Ca2+]i from the baseline was attenuated in P1 (96.6 +/- 6.9%) and P2 (96.1 +/- 5.4%) compared with HRC (143.8 +/- 11.5%), (P < 0.001), with no difference between P1 and P2. The expression of TNF-alpha mRNA increased only in HRC during hypoxia-reoxygenation. KC viability increased in P1 (97.5 +/- 2.6%) and P2 (94.6 +/- 2.9%), compared with HRC (89.9 +/- 1.4%), (P < 0.005), with no difference between P1 and P2.

CONCLUSION: The results indicate that propofol attenuates KC activation and protects KC from hypoxia-reoxygenation injury at clinically relevant concentrations. This attenuation seems to result from inhibition of [Ca2+]i increase in KC.
Department
Dept. of Physiology (생리학)
Publisher
School of Medicine
Citation
Eon-Gi Sung et al. (2005). Propofol attenuates Kupffer cell activation during hypoxia-reoxygenation
[Le propofol diminue ľactivation des cellules de Kupffer pendant ľhypoxie-réoxygénation]. Canadian Journal of Anesthesia, 52(9), 921–926. doi: 10.1007/BF03022052
Type
Article
ISSN
0832-610X
DOI
10.1007/BF03022052
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35276
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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