계명대학교 의학도서관 Repository

Propofol attenuates Kupffer cell activation during hypoxia-reoxygenation [Le propofol diminue ľactivation des cellules de Kupffer pendant ľhypoxie-réoxygénation]

Metadata Downloads
Author(s)
Eon-Gi SungDaelim JeeIn-Hwan SongHee-Sun KimJae Hoon BaeSe-Hun Park
Publication Year
2005
Abstract
PURPOSE: We undertook a study to determine whether propofol may attenuate Kupffer cell (KC) activation, thus protecting the cells against hypoxia-reoxygenation injury through the modulation of intracellular calcium ([Ca2+]i).

METHODS: [Ca2+]i, the expression of tumour necrosis factor (TNF)-alpha mRNA, and KC viability were measured in response to hypoxia-reoxygenation following pretreatment with propofol 0.5 and 5 microg.mL(-1) (Groups P1 and P2, respectively) or without propofol (Group HRC). KCs were isolated and cultured from male Sprague-Dawley rats. KCs were incubated under an atmosphere of hypoxia (95% N2 + 5% CO2) for 60 min with subsequent 120 min reoxygenation (95% air + 5% CO2). [Ca2+]i for the first 12 min after reoxygenation, TNF-alpha mRNA, and KC viability at the end of reoxygenation in groups P1 and P2 were compared with those of HRC.

RESULTS: The increase of [Ca2+]i from the baseline was attenuated in P1 (96.6 +/- 6.9%) and P2 (96.1 +/- 5.4%) compared with HRC (143.8 +/- 11.5%), (P < 0.001), with no difference between P1 and P2. The expression of TNF-alpha mRNA increased only in HRC during hypoxia-reoxygenation. KC viability increased in P1 (97.5 +/- 2.6%) and P2 (94.6 +/- 2.9%), compared with HRC (89.9 +/- 1.4%), (P < 0.005), with no difference between P1 and P2.

CONCLUSION: The results indicate that propofol attenuates KC activation and protects KC from hypoxia-reoxygenation injury at clinically relevant concentrations. This attenuation seems to result from inhibition of [Ca2+]i increase in KC.
Department
Dept. of Physiology (생리학)
Publisher
School of Medicine
Citation
Canadian Journal of Anesthesia, Vol.52(9) : 921-926, 2005
Type
Article
ISSN
0832-610X
DOI
10.1007/BF03022052
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35276
Authorize & License
  • AuthorizeOpen
  • EmbargoForever
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.