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Failure to activate caspase 3 in phorbol ester-resistant leukemia cells is associated with resistance to apoptotic cell death

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Author(s)
Yun-Jung ChoiJong-Wook ParkJu-Hyung WooYoung-Ho KimSang-Han LeeJin-Man LeeTaeg Kyu Kwon
Publication Year
2002
Abstract
The protein kinase C (PKC)-specific inhibitor, Ro-31-8220, has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. In the present study, we determined the molecular pathways that lead to apoptosis after treatment of cells with the PKC-specific inhibitor RO-31-8220. For this, we used the U937 human leukemia cell line and a phorbolmyristate acetate (PMA)-resistant derivative cell line, R-U937. Ro-31-8220 treatment of U937 cells leads to apoptosis, which is accompanied by activation of caspase 3 (as measured by decreased levels of the 32 kDa inactive form and increased proteolytic cleavage of phospholipase C (PLC)-γ1). The broad-range caspase inhibitor z-VAD-fmk inhibits this induction of apoptosis, supporting a direct link between caspase activation and Ro-31-8220 induction of apoptosis. This activation of apoptosis is also accompanied by release of cytochrome c, but not by altered expression of Bcl-2 family protein or IAP family proteins. In R-U937 cells, Ro-31-8220 fails to cause release of cytochrome c, activation of caspase 3, or apoptosis. Activation of Akt occurs to a greater extent in the R-U937 cells than the U937 cells and thus might be related to protection from Ro-31-8220-induced apoptosis.

Keywords
Protein kinase C inhibitor;
Cell cycle;
Apoptosis;
Caspase 3;
Akt
Department
Dept. of Immunology (면역학)
Publisher
School of Medicine
Citation
Cancer Letters, Vol.182(2) : 183-191, 2002
Type
Article
ISSN
0304-3835
DOI
10.1016/S0304-3835(02)00082-4
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35316
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