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Failure to induce inhibition of cyclin A and up-regulation of p21 expression in phorbol ester-resistant U937 cells by phorbol ester

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Author(s)
권택규박종욱서민호서성일백원기
Alternative Author(s)
Kwon, Taeg KyuPark, Jong WookSuh, Min HoSuh, Seong IlBaek, Won Ki
Publication Year
2000
Abstract
Differentiation resistant U937 cells were derived from parental U937 cells by selecting for continuously growing U937 cells in cell cultures continuously exposed to phorbol 12 myristate 13-acetate (PMA). Unlike in other known PMA resistant U937, the basal expression of protein kinase C (PKC) isozymes in these PMA resistant cells (R-U937) was significantly decreased. Subsequent analyses revealed differences between the wild type U937 and the R-U937 cells with respect to G1 phase arrest, which seemed to occur in U937 because of low levels of cdk2 kinase activity. This abolished cdk2 kinase activity is mainly due to inhibition of cdk2 phosphorylation, cyclin A down-regulation and cyclin dependent kinase inhibitor p21 up-regulation. Our data suggest that events down-stream of PKC activation may mediate cell cycle control. Thus, the R-U937 cells could be useful for further PKC mediated cell cycle control studies.

Keywords : Cell cycle; Cyclin dependent kinase; p21; Phorbol ester; Protein kinase C
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
Cancer Letters, Vol.157(2) : 211-218, 2000
Type
Article
ISSN
0304-3835
DOI
10.1016/S0304-3835(00)00492-4
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35319
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