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Lack of mutation at p16INK4A gene but expression of aberrant p16INK4A RNA transcripts in human ovarian carcinoma

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Author(s)
서성일백원기서민호
Alternative Author(s)
Suh, Seong IlBaek, Won KiSuh, Min Ho
Publication Year
2000
Abstract
Alterations of the p16INK4A gene are frequent in various human cancers. We investigated p16INK4A gene status in 20 ovarian carcinomas by PCR (polymerase chain reaction), PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and sequencing techniques. None of the primary tumors showed any mutational or deletional events. However, 19 out of 20 tumors displayed both a methylated and an unmethylated p16INK4A promoter. In some of these samples, we detected aberrant p16INK4A transcripts, with partial deletions of both exons 1 and 2, which could not encode a functional p16INK4A protein. The sequences of the aberrant mRNA revealed common 4–7 nucleotide sequences before and after the deleted region, which might cause abnormal splicing of mRNA transcripts. These results suggest that both promoter methylation and aberrant mRNA processing may interfere with p16INK4A expression in ovarian tumors.

Keywords
Aberrant p16INK4A transcripts;
Methylation;
Ovarian carcinoma
Department
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
Cancer Letters, Vol.153(1-2) : 175-182, 2000
Type
Article
ISSN
0304-3835
DOI
10.1016/S0304-3835(00)00369-4
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35321
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