Transglutaminase 2 expression levels regulate sensitivity to cystamine plus TRAIL-mediated apoptosis

Authors
Ji Hoon JangJun Soo ParkTae-Jin LeeTaeg Kyu Kwon
Department
Dept. of Immunology (면역학)
Issue Date
2010
Citation
Cancer Letters, Vol.287(2) : 224-230, 2010
ISSN
0304-3835
Abstract
In this study, we demonstrate for the first time that cystamine, an inhibitor of transglutaminase 2 (TG2), enhanced tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in Caki cells, but not in normal human mesangial cells. Cystamine plus TRAIL-induced down-regulation of c-FLIP was recovered to basal levels by addition of the pancaspase inhibitor, z-VAD. The forced expression of c-FLIP attenuated cystamine plus TRAIL-mediated apoptosis in Caki cells. Although, cells expressing high levels of TG2 were more sensitive to cystamine plus TRAIL-mediated apoptosis than were cells expressing low levels of TG2, cystamine plus TRAIL-mediated apoptosis in the cell line expressing high levels of TG2 was reduced when TG2 levels were knocked down with siRNA. These results indicate that the level of TG2 modulates cystamine plus TRAIL-induced apoptosis. Taken together, the present findings suggest that cystamine may be an effective sensitizer of TRAIL-induced apoptosis in cancer cells expressing high levels of TG2. Keywords TRAIL; Cystamine; c-FLIP; Apoptosis; Transglutaminase 2
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35326
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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Full Text
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0304383509004285?via%3Dihub
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