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Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity

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Affiliated Author(s)
서성일백원기서민호박종욱권택규
Alternative Author(s)
Suh, Seong IlBaek, Won KiSuh, Min HoPark, Jong WookKwon, Taeg Kyu
Journal Title
Carcinogenesis
ISSN
0143-3334
Issued Date
2001
Abstract
Resveratrol has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. In the present study, we determined the effect of high intracellular levels of the anti-apoptosis protein Bcl-2 on caspase-3 activation, PLC-γ1 degradation and cytochrome c release during resveratrol-induced apoptosis. For this, we used U937/vector and U937/Bcl-2 cells, which were generated by transfection of the cDNA of the Bcl-2 gene. As compared with U937/vector, U937/Bcl-2 cells exhibited a 4-fold greater expression of Bcl-2. Treatment with 60 or 100 μM resveratrol for 24 h produced morphological features of apoptosis and DNA fragmentation in U937/vector cells, respectively. This was associated with caspase-3 activation and PLC-γ1 degradation. In contrast, resveratrol-induced caspase-3 activation and PLC-γ1 degradation and apoptosis were significantly inhibited in U937/Bcl-2 cells. Bcl-2 overexpressing cells exhibited less cytochrome c release and sustained expression levels of the IAP proteins during resveratrol-induced apoptosis. In addition, these findings indicate that Bcl-2 inhibits resveratrol-induced apoptosis by a mechanism that interferes with cytochrome c release and activity of caspase-3 that is involved in the execution of apoptosis.
Department
Dept. of Microbiology (미생물학)
Dept. of Immunology (면역학)
Publisher
School of Medicine
Citation
Jong-Wook Park et al. (2001). Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity. Carcinogenesis, 22(10), 1633–1639. doi: 10.1093/carcin/22.10.1633
Type
Article
ISSN
0143-3334
DOI
10.1093/carcin/22.10.1633
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35353
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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