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Protective Role of Glucagon-like Peptide-1 Against Glucosamine-induced Cytotoxicity in Pancreatic Beta Cells

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Author(s)
박재형송대규백원기서성일임정근
Alternative Author(s)
Park, Jae HyungSong, Dae KyuBaek, Won KiSuh, Seong IlLim, Jeong Geun
Publication Year
2010
Abstract
High doses of glucosamine have been known to induce apoptosis of pancreatic beta cells. The mechanism for this phenomenon has not been clearly elucidated. We aimed to explore the potential mechanisms for glucosamine toxicity in the rat insulinoma cell line INS-1 and in rat native beta cells. We also investigated whether glucagon-like peptide (GLP)-1 could be protective against glucosamine. Glucosamine exhibited dose-dependent inhibition of cell survival and an increase in the cell population at the sub-G1 phase. Glucosamine was revealed to inhibit cellular glucose uptake, resulting in the activation of AMP-activated protein kinase (AMPK). Accordingly, phosphorylation of P70S6K and ribosomal protein S6 (S6RP) was decreased. Protein glycosylation appeared not to be involved in this cytotoxicity. Pretreatment with GLP-1 alleviated glucosamine-mediated inhibition of glucose uptake and lessened AMPK activation, thus allowing recovery of the phosphorylation levels of P70S6K and S6RP. The effect of GLP-1 was blocked by the adenylyl cyclase inhibitor MDL12330A but not by the protein kinase A inhibitor H89. Taken together, these data demonstrate that glucosamine may inhibit beta-cell survival by diminishing cellular glucose uptake independent of glycosylation. This glucosamine toxicity can be blocked by GLP-1, which leads to recovery of the glucose uptake through a PKA-independent, cAMP-dependent mechanism.
Department
Dept. of Physiology (생리학)
Dept. of Microbiology (미생물학)
Dept. of Neurology (신경과학)
Publisher
School of Medicine
Citation
Cellular Physiology and Biochemistry, Vol.25(2-3) : 211-220, 2010
Type
Article
ISSN
1015-8987
DOI
10.1159/000276555
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35386
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