계명대학교 의학도서관 Repository

Withaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition

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Affiliated Author(s)
권택규김진희
Alternative Author(s)
Kwon, Taeg KyuKim, Jin Hee
Journal Title
Chemico-Biological Interactions
ISSN
0009-2797
Issued Date
2011
Abstract
Withaferin A (Wit A), a natural compound derived from the medicinal plant Withania somnifera, has been reported for the anti-tumor effects, including the inhibition of tumor cell growth, metastasis and angiogenesis. In this study, we investigated the effect of Wit A on radiation-induced apoptosis in human renal cancer cells (Caki cells). Our results showed that, compared with Wit A or radiation alone, the combination of both resulted in a significant enhancement of apoptosis, showing the increase in the cleavage of caspase-3 and PARP as well as sub-G1 cell population. In addition, reactive oxygen species (ROS) generation was correlated with the enhancement of radiation-induced apoptosis by Wit A. Wit A downregulated Bcl-2 protein levels and ectopic expression of Bcl-2 in Caki cells attenuated the apoptosis induced by Wit A plus radiation. Taken together, these results indicate that Wit A enhanced radiation-induced apoptosis in Caki cells through ROS generation, down-regulation of Bcl-2 and Akt dephosphorylation. Thus, our study shows that Wit A may be used as an effective radiosensitizer in cancer therapy.

Keywords
Withaferin A;
Radiation;
Apoptosis;
Reactive oxygen species;
Bcl-2
Department
Dept. of Immunology (면역학)
Dept. of Radiation Oncology (방사선종양학)
Publisher
School of Medicine
Citation
Eun Sun Yang et al. (2011). Withaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition. Chemico-Biological Interactions, 190(1), 9–15. doi: 10.1016/j.cbi.2011.01.015
Type
Article
ISSN
0009-2797
DOI
10.1016/j.cbi.2011.01.015
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35396
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학)
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