INHIBITORY EFFECTS OF GLUCOSAMINE ON
LIPOPOLYSACCHARIDE-INDUCED ACTIVATION IN
MICROGLIAL CELLS
- Affiliated Author(s)
- 이현아; 이상도; 송대규; 배재훈; 신동훈; 서성일; 김상표; 문교철; 장병철
- Alternative Author(s)
- Yi, Hyon Ah; Yi, Sang Do; Song, Dae Kyu; Bae, Jae Hoon; Shin, Dong Hoon; Suh, Seong Il; Kim, Sang Pyo; Mun, Kyo Cheol; Jang, Byeong Churl
- Journal Title
- Clinical and Experimental Pharmacology and Physiology
- ISSN
- 0305-1870
- Issued Date
- 2005
- Abstract
- SUMMARY
1. The aim of the present study was to investigate the effects
of glucosamine on lipopolysaccharide (LPS)-induced cellular
activation in microglia and to evaluate the inhibitory
mechanisms involved.
2. Lipopolysaccharide (100 ng/mL) was used for the
activation of primary cultured rat microglial or BV2 microglial
cells. Changes in intracellular Ca
2+
levels and outward K
+
currents were measured using fura-2/AM and whole-cell
patch-clamp methods, respectively. Lipopolysaccharide-
induced expression of tumour necrosis factor (TNF)-
mRNA
was analysed by reverse transcription–polymerase chain
reaction.
3. Lipopolysaccharide transformed cell morphology into an
amoeboid shape
in
vitro
and induced microglial activation
in vivo
, as measured by immunohistochemical staining, but
glucosamine inhibited this activation. Glucosamine also
inhibited LPS-induced Ca
2+
influx, outward K
+
currents and
TNF-
mRNA expression, which are typically representative
of microglial activation.
4. The results suggest that the inhibitory mechanisms of
glucosamine on LPS-induced microglial activation include
inhibition of Ca
2+
influx and outward K
+
currents, as well as
downregulation of the microglial activator gene TNF-
.
Key words: glucosamine, lipopolysaccharide, microglial
activation.
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