계명대학교 의학도서관 Repository

A New Method of Harvesting Descemet Membrane–Endothelium Complex Approaching From the Suprachoroidal Space for Descemet Membrane Endothelial Keratoplasty: An Experimental Animal Study

Metadata Downloads
Author(s)
Sung Dong ChangJong Hwa JunKwon Ho Hong
Publication Year
2010
Abstract
Purpose: To report the study in rabbits of a new method of
harvesting Descemet membrane–endothelium complex approaching
from the suprachoroidal space for Descemet membrane endothelial
keratoplasty.
Materials and Methods: Twenty-four eyes of 12 rabbits were
used for microscopic analysis, whereas 24 eyes of 12 cats were used
for gross photographic analysis. The enucleated eyeballs of the
rabbits and cats were incised 360 degrees circumferentially along
the equator. Manual separation was performed between the sclera
and the underlying connective tissue approaching from the suprachoroidal
space in the direction of the central cornea step by step in
spiral pattern. After complete separation of the stroma from the
Descemet membrane–endothelium–iris complex, it was excised from
the globe at the outside of the iris root. The synthetic carrier (soft
contact lens) was laid down on the punch block, and then the excised
complex was brought over the carrier with the endothelium–iris side
upward. Two steps of down punch for the designed graft size were
performed. The harvested complexes were processed for vital stains
and were evaluated through photography taken under a light microscope
and gross close-up. The endothelial cell loss rates were calculated
by a computer software.
Results: The overall harvest success rate was 89.6% (43 of 48). The
overall structural cell loss rate was 1.83 6 2.34 by microscopic
analysis and 4.68% 6 1.68% by gross photographic analysis.
Conclusion: The new method of en bloc harvesting Descemet
membrane–endothelium complex approaching from the suprachoroidal
space could provide minimal damage to the endothelium during
harvesting, and it can be carried and passed through an injector.
Key Words: endothelial keratoplasty, Descemet membrane endothelial
keratoplasty, corneal transplantation
Department
Dept. of Ophthalmology (안과학)
Publisher
School of Medicine
Citation
Cornea, Vol.29(8) : 904-909, 2010
Type
Article
ISSN
0277-3740
DOI
10.1097/ICO.0b013e3181c6b637
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35535
Authorize & License
  • AuthorizeOpen
  • EmbargoForever
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.