Assignment of BUB3 to human chromosome band 10q26 by in situ hybridization

Abstract

Rationale and significance Eukaryotic organisms have developed mitotic checkpoints to inhibit cell division when the mitotic spindle function is damaged and to assure accurate segregation of replicated chromosomes. Altered mitotic checkpoints in neoplasia may contribute to the genomic instability that is characteristic of cancerous cells (Elledge, 1998; Hardwick, 1998; Lengauer et al., 1998). Among these checkpoint proteins is Bub3, which is a kinase substrate for Bub1 and is necessary for Bub1 to bind to kinetochores before chromosome alignment (Taylor et al., 1998). Recently, the cDNA sequence corresponding to the human homologue of the S. cerevisiae Bub3 mitotic checkpoint protein has been described and about 34% of the amino acid sequences are shared between S. cerevisiae and human proteins (Taylor et al., 1998). In the present study, we determined the chromosome location of the human BUB3 gene to be 10q26, a locus that is frequently affected by chromosome loss in human neoplasia