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Low sensitivity of periprosthetic tissue PCR for prosthetic knee infection diagnosis

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Affiliated Author(s)
류성열
Alternative Author(s)
Ryu, Seong Yeol
Journal Title
Diagnostic Microbiology and Infectious Disease
ISSN
0732-8893
Issued Date
2014
Abstract
We previously described a rapid PCR panel targeting bacteria associatedwith prosthetic joint infection (PJI) for use
on biofilms dislodged from explanted hip and knee arthroplasties. Herein, we tested the PCR panel on
periprosthetic tissues from95 subjects undergoing resection knee arthroplasty, all ofwhomhad had tissue cultures
performed. A subset also had synovial fluid culture (n = 89) and/or device vortexing and sonication with culture
(n = 58), and a subset of these PCR (n = 36) of resulting sonicate fluid, performed. Of the 64 subjectswith PJI, 10
and 44 had positive tissue PCR and cultures, respectively, one of whom had a positive tissue PCR with negative
tissue cultures. The overall sensitivity of tissue PCR (16%) was much lower than that of tissue (69%), synovial fluid
(72%) and sonicate fluid (77%) culture aswell as sonicate fluid PCR (78%) (P b 0.00001, P b 0.00001, P b 0.00001, and
P = 0.0003, respectively). Periprosthetic tissue PCR, as performed herein, has poor sensitivity for PJI diagnosis
Department
Dept. of Internal Medicine (내과학)
Publisher
School of Medicine
Citation
Seong Yeol Ryu et al. (2014). Low sensitivity of periprosthetic tissue PCR for prosthetic knee
infection diagnosis. Diagnostic Microbiology and Infectious Disease, 79(4), 448–453. doi: 10.1016/j.diagmicrobio.2014.03.021
Type
Article
ISSN
0732-8893
DOI
10.1016/j.diagmicrobio.2014.03.021
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35588
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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