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Phospholipase C, protein kinase C, Ca2+/calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells

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Affiliated Author(s)
권택규
Alternative Author(s)
Kwon, Taeg Kyu
Journal Title
European Journal of Biochemistry
ISSN
0014-2956
Issued Date
2004
Abstract
We show that epigallocatechin-3 gallate (EGCG), a major
component of green tea, stimulates phospholipase D (PLD)
activity in U87 human astroglioma cells. EGCG-induced
PLD activation was abolished by the phospholipase C
(PLC) inhibitor and a lipase inactive PLC-c1mutant,which
is dependent on intracellular or extracellular Ca2+, with the
possible involvement of Ca2+/calmodulin-dependent protein
kinase II (CaM kinase II). EGCGinduced translocation
of PLC-c1 from the cytosol to the membrane and PLC-c1
interactionwith PLD1.EGCGregulates the activity of PLD
by modulating the redox state of the cells, and antioxidants
reverse this effect. Moreover, EGCG-induced PLD
activation was reduced by PKC inhibitors or down-regulation
of PKC. Taken together, these results show that, in
human astroglioma cells, EGCG regulates PLD activity
via a signaling pathway involving changes in the redox state
that stimulates a PLC-c1 [Ins(1,4,5)P3-Ca2+]–CaM kinase
II–PLDpathway and a PLC-c1 (diacylglycerol)–PKC–PLD
pathway.
Keywords: Ca2+/calmodulin-dependent protein kinase II;
epigallocatechin-3 gallate; phospholipase C-c1; phospholipase
D; reactive oxygen species.
Department
Dept. of Immunology (면역학)
Publisher
School of Medicine
Citation
Shi Yeon Kim et al. (2004). Phospholipase C, protein kinase C, Ca2+/calmodulin-dependent
protein kinase II, and redox state are involved in epigallocatechin
gallate-induced phospholipase D activation in human astroglioma cells. European Journal of Biochemistry, 271(17), 3470–3480. doi: 10.1111/j.0014-2956.2004.04242.x
Type
Article
ISSN
0014-2956
DOI
10.1111/j.0014-2956.2004.04242.x
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35651
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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