계명대학교 의학도서관 Repository

Curcumin inhibits the expression of COX-2 in UVB-irradiated human keratinocytes (HaCaT) by inhibiting activation of AP-1: p38 MAP kinase and JNK as potential upstream targets

Metadata Downloads
Affiliated Author(s)
조재위김창욱이규석장병철백원기서민호서성일
Alternative Author(s)
Cho, Jae WeKim, Chang WookLee, Kyu SukJang, Byeong ChurlBaek, Won KiSuh, Min HoSuh, Seong Il
Journal Title
Experimental and Molecular Medicine.
ISSN
1226-3613
Issued Date
2005
Abstract
Ultraviolet B (UVB) irradiation of skin induces an
acute inflammation. Cyclooxygenase-2 (COX-2)
protein plays key roles in acute inflammation in
UVB-irradiated keratinocyte cell line HaCaT. Recently,
curcumin has been regarded as a promising
anti-inflammatory agent due to its ability
to inhibit COX-2 expression. However, it remains
largely unknown whether curcumin inhibits the
UVB-induced COX-2 expression in HaCaT cells.
This study was undertaken to clarify the effect
of curcumin on the expression of COX-2 in UVBirradiated
HaCaT cells and further determined the
molecular mechanisms associated with this process.
In this study, we have found that the expression
of COX-2 mRNA and protein were up-regulated
in UVB-irradiated HaCaT cells in a doseand
time-dependent manner. Interestingly, treatment
with curcumin strongly inhibited COX-2
mRNA and protein expressions in UVB-irradiated
HaCaT cells. Notably, there was effective inhibition
by curcumin on UVB-induced activations of
p38 MAPK and JNK in HaCaT cells. The DNA
binding activity of AP-1 transcription factor was
also markedly decreased with curcumin treatment
in UVB-irradiated HaCaT cells. These results collectively
suggest that curcumin may inhibit COX-
2 expression by suppressing p38 MAPK and JNK
activities in UVB-irradiated HaCaT cells. We propose
that curcumin may be applied as an effective
and novel sunscreen drug for the protection
of photoinflammation.
Keywords: COX-2; curcumin; HaCaT; MAPK; UVB
Department
Dept. of Dermatology (피부과학)
Dept. of Molecular Medicine (분자의학)
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
Publisher
School of Medicine
Citation
Jae-We Cho et al. (2005). Curcumin inhibits the expression of COX-2 in UVB-irradiated
human keratinocytes (HaCaT) by inhibiting activation of AP-1:
p38 MAP kinase and JNK as potential upstream targets. Experimental and Molecular Medicine., 37(3), 186–192. doi: 10.1038/emm.2005.25
Type
Article
ISSN
1226-3613
DOI
10.1038/emm.2005.25
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35705
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.