Curcumin inhibits the expression of COX-2 in UVB-irradiated
human keratinocytes (HaCaT) by inhibiting activation of AP-1:
p38 MAP kinase and JNK as potential upstream targets
- Affiliated Author(s)
- 조재위; 김창욱; 이규석; 장병철; 백원기; 서민호; 서성일
- Alternative Author(s)
- Cho, Jae We; Kim, Chang Wook; Lee, Kyu Suk; Jang, Byeong Churl; Baek, Won Ki; Suh, Min Ho; Suh, Seong Il
- Journal Title
- Experimental and Molecular Medicine.
- ISSN
- 1226-3613
- Issued Date
- 2005
- Abstract
- Ultraviolet B (UVB) irradiation of skin induces an
acute inflammation. Cyclooxygenase-2 (COX-2)
protein plays key roles in acute inflammation in
UVB-irradiated keratinocyte cell line HaCaT. Recently,
curcumin has been regarded as a promising
anti-inflammatory agent due to its ability
to inhibit COX-2 expression. However, it remains
largely unknown whether curcumin inhibits the
UVB-induced COX-2 expression in HaCaT cells.
This study was undertaken to clarify the effect
of curcumin on the expression of COX-2 in UVBirradiated
HaCaT cells and further determined the
molecular mechanisms associated with this process.
In this study, we have found that the expression
of COX-2 mRNA and protein were up-regulated
in UVB-irradiated HaCaT cells in a doseand
time-dependent manner. Interestingly, treatment
with curcumin strongly inhibited COX-2
mRNA and protein expressions in UVB-irradiated
HaCaT cells. Notably, there was effective inhibition
by curcumin on UVB-induced activations of
p38 MAPK and JNK in HaCaT cells. The DNA
binding activity of AP-1 transcription factor was
also markedly decreased with curcumin treatment
in UVB-irradiated HaCaT cells. These results collectively
suggest that curcumin may inhibit COX-
2 expression by suppressing p38 MAPK and JNK
activities in UVB-irradiated HaCaT cells. We propose
that curcumin may be applied as an effective
and novel sunscreen drug for the protection
of photoinflammation.
Keywords: COX-2; curcumin; HaCaT; MAPK; UVB
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