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Protective effect of Codium fragile against UVB-induced pro-inflammatory and oxidative damages in HaCaT cells and BALB/c mice

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Affiliated Author(s)
장정희
Alternative Author(s)
Jang, Jung Hee
Journal Title
Fitoterapia
ISSN
0367-326X
Issued Date
2013
Abstract
Acute exposure to ultraviolet (UV) radiation causes pro-inflammatory responses via diverse
mechanisms including oxidative stress. Codium fragile is a green alga of Codiales family and has
been reported to exhibit anti-edema, anti-allergic, anti-protozoal and anti-mycobacterial
activities. In this study, we have investigated a novel anti-inflammatory potential of C. fragile
using in vitro cell culture as well as in vivo animal models. In HaCaT cells, buthanol and
ethylacetate fractions of 80% methanol C. fragile extract (CFB or CFE) and a single compound,
clerosterol (CLS) isolated from CFE attenuated UVB (60 mJ/cm2)-induced cytotoxicity and
reduced expression of pro-inflammatory proteins including cyclooxygenase-2 (COX-2),
inducible nitric oxide synthase (iNOS), and tumor necrosis factor-α (TNF- α). Moreover,
CFB, CFE and CLS effectively suppressed UVB-induced production of pro-inflammatory
mediators such as prostaglandin E2 (PGE2) and nitric oxide (NO). In another experiment,
topical application of CFB, CFE or CLS prior to UVB irradiation (200 mJ/cm2) on BALB/c mice,
inhibited the UVB-elevated protein levels of COX-2, iNOS, and TNF-α. Furthermore, CFB, CFE and
CLS suppressed oxidative damages caused by UVB irradiation for example lipid peroxidation and/
or protein carbonylation, which seemed to be mediated by up-regulation of antioxidant defense
enzymes. These results suggest that C. fragile could be an effective therapeutic agent providing
protection against UVB-induced inflammatory and oxidative skin damages.
Department
Dept. of Pharmacology (약리학)
Publisher
School of Medicine
Citation
Chan Lee et al. (2013). Protective effect of Codium fragile against UVB-induced
pro-inflammatory and oxidative damages in HaCaT cells
and BALB/c mice. Fitoterapia, 86, 54–63. doi: 10.1016/j.fitote.2013.01.020
Type
Article
ISSN
0367-326X
DOI
10.1016/j.fitote.2013.01.020
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35742
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
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