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Immune response induced by immunization with Hepatitis B virus core DNA isolated from chronic active hepatitis patients

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Affiliated Author(s)
권택규박종욱서민호서성일백원기
Alternative Author(s)
Kwon, Taeg KyuPark, Jong WookSuh, Min HoSuh, Seong IlBaek, Won Ki
Journal Title
Immunology letters
ISSN
0165-2478
Issued Date
2001
Abstract
There are many mutations in the gene encoding Hepatitis B virus (HBV) core antigen of chronic active hepatitis patients, and
such mutations are most likely to be related to the severity of disease. Here, we constructed plasmids containing wild-type and
deletion type of HBV core gene (HBc) to develop an experimental DNA vaccine and to compare immunogenicity of two types
of HBc vaccine. Twenty-nine wild-types and seven deletion types of HBc were detected in sera of 32 Korean patients with chronic
active hepatitis. Four wild-types (W1, W2, W4, W6) and two deletion types (D3, D4) of HBc were cloned into the pcDNA3
vector. Intramuscular immunization with wild-type HBc efficiently increased serum anti-HBc antibody response in a dose-dependent
manner. Anti-HBc antibody response in mice injected with W6 increased 14 days after immunization, and peaked after 30
days and was maintained at least up to 50 days. W6 immunization induced a specific cytotoxic T lymphocyte response to
W6-transfected 3LL (3LL-W6), and reduced the sizes of tumor mass of mice challenged with 3LL-W6 or 3LL transfected with
D4. However, intramuscular immunization with D3 and D4 did not show antibody response at all. D3 and D4 have 157 bp (from
331 to 491 bp) and 122 bp (from 327 to 448 bp) gene deletion, respectively, and these encode class II MHC-restricted T-cell
epitope. Altogether, these results suggest that mutant virus that has deleted HBc gene may evade immune systems due to loss of
T-cell epitope. © 2001 Elsevier Science B.V. All rights reserved.
Keywords: Hepatitis B virus; Core gene; DNA vaccine; CTL (cytotoxic T lymphocyte)
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
Young-Sun Lee et al. (2001). Immune response induced by immunization with Hepatitis B virus
core DNA isolated from chronic active hepatitis patients. Immunology letters, 78(1), 13–20. doi: 10.1016/S0165-2478(01)00230-9
Type
Article
ISSN
0165-2478
DOI
10.1016/S0165-2478(01)00230-9
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35854
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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