Exogenous wild-type p16INK4A gene induces delayed cell proliferation and promotes chemosensitivity through decreased pRB and increased E2F-1 expressions

Authors
Yong-Wook JeongKi-Sung KimJae-Young OhJong-Chun ParkWon-Ki BaekSeong-Il SuhMin-Ho SuhJe-Chul LeeJae-We Cho
Department
Dept. of Microbiology (미생물학); Dept. of Dermatology (피부과학)
Issue Date
2003
Citation
International Journal of Molecular Medicine, Vol.12(1) : 61-65, 2003
ISSN
1107-3756
Abstract
Human gastric cancer SNU 484 cells express mutant p16, which migrates slower than the wild-type p16. We constructed an expression vector containing human p16 cDNA to evaluate the cytotoxic effects of exogenous p16 expression on SNU 484 cell proliferation and to explore the potential use of p16 in cancer gene therapy. The stable transfectant expressing wild-type p16, showed a 2-fold slower growth rate than mock and non-infected cells through down-regulation of CDK4-dependent kinase activity. When cells were transiently transfected with mock or p16 encoded vector, the mock cells showed larger survival colonies than those of wild-type p16. Furthermore, p16-expressing stable transfectant was readily progressed into cell death by combination with treatment of chemotherapeutic drug in a dose-dependent manner. According to western blot analysis, both decreased expression of pRB and increased expression of E2F-1 may contribute to the susceptibility of cell death. Our data indicate that exogenous wild-type p16 induces delayed cell proliferation and promotes chemo-sensitivity in the gastric cancer cell line, implying the promise of p16 in cancer gene therapy.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35910
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Keimyung Author(s)
백원기; 서성일; 서민호; 조재위
Full Text
https://www.spandidos-publications.com/ijmm/12/1/61
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