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Platelet-derived growth factor-D induces expression of cyclooxygenase-2 in rat mesangial cells through activation of PI3K/PKB and PKCs

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Affiliated Author(s)
장병철최미선
Alternative Author(s)
Jang, Byeong ChurlChoe, Mi Sun
Journal Title
International Journal of Molecular Medicine
ISSN
1107-3756
Issued Date
2013
Abstract
. Platelet-derived growth factor (PDGF)-D is suggested to be a key factor in the development of several renal pathologies, including mesangioproliferative glomerulonephritis. Cyclooxygenase (COX)-2 is a protein involved in the biosynthesis of inflammatory prostaglandins. In this study, we investigated the effect of PDGF-D on the regulation of COX-2 expression in rat mesangial cells (RMCs). Treatment with PDGF-D induced COX-2 at both the protein and mRNA levels in RMCs, suggesting that the PDGF-D-mediated induction of COX-2 is due to COX-2 transcriptional upregulation. PDGF-D treatment also led to a rapid but transient activation of PKB and extracellular signal regulated kinase (ERK)-1/2. Activities of JNK-1/2 and p38 MAPK, however, were not influenced by PDGF-D in RMCs. Markedly, pharmacological inhibition studies showed that pretreatment with LY294002 (a PI3K/PKB inhibitor) or GF109203X (a pan-PKC inhibitor) suppressed the PDGF-D-induced expression of COX-2 protein and mRNA, while pretreatment with PD98059 (an ERK-1/2 inhibitor) or PP1 (an Src inhibitor) had no effect on it. These findings collectively demonstrate for the first time that PDGF-D induces COX-2 by transcriptional upregulation in RMCs and the induction is largely related to PI3K/PKB and PKCs activities.
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Pathology (병리학)
Publisher
School of Medicine
Citation
YU-KYOUNG PARK et al. (2013). Platelet-derived growth factor-D induces expression of
cyclooxygenase-2 in rat mesangial cells through activation of PI3K/PKB and PKCs. International Journal of Molecular Medicine, 31(2), 447–452. doi: 10.3892/ijmm.2012.1216
Type
Article
ISSN
1107-3756
DOI
10.3892/ijmm.2012.1216
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35916
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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