Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type Ⅰ collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess

Authors
DO HYUN LEEKYUNG-HA CHOIJAE-WE CHOSO YOUNG KIMTAE RIN KWONSUN YOUNG CHOIYOO MI CHOIJAY LEEHO SANG YOONBEOM JOON KIM
Department
Dept. of Dermatology (피부과학)
Issue Date
2014
Citation
International Journal of Molecular Medicine, Vol.33(5) : 1147-1152, 2014
ISSN
1107-3756
Abstract
. Application of growth factor mixtures has been used for wound healing and anti-wrinkles agents. The aim of this study was to evaluate the effect of recombinant growth factor mixtures (RGFM) on the expression of cell cycle regulatory proteins, type I collagen, and wound healing processes of acute animal wound models. The results showed that RGFM induced increased rates of cell proliferation and cell migration of human skin fibroblasts (HSF). In addition, expression of cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)4, and Cdk2 proteins was markedly increased with a growth factor mixtures treatment in fibroblasts. Expression of type I collagen was also increased in growth factor mixtures-treated HSF. Moreover, growth factor mixtures-induced the upregulation of type I collagen was associated with the activation of Smad2/3. In the animal model, RGFM-treated mice showed accelerated wound closure, with the closure rate increasing as early as on day 7, as well as re-epithelization and reduced inflammatory cell infiltration than phosphate-buffered saline (PBS)-treated mice. In conclusion, the results indicated that RGFM has the potential to accelerate wound healing through the upregulation of type I collagen, which is partly mediated by activation of Smad2/3-dependent signaling pathway as well as cell cycle progression in HSF. The topical application of growth factor mixtures to acute and chronic skin wound may accelerate the epithelization process through these molecular mechanisms.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35920
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Keimyung Author(s)
조재위
Full Text
https://www.spandidos-publications.com/ijmm/33/5/1147
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