Platelet-rich plasma induces increased expression of G1 cell cycle regulators, type Ⅰ collagen, and matrix metalloproteinase-1 in human skin fibroblasts

Authors
JAE-WE CHOSUNG-AE KIMKYU-SUK LEE
Department
Dept. of Dermatology (피부과학)
Issue Date
2012
Citation
International Journal of Molecular Medicine, Vol.29(1) : 32-36, 2012
ISSN
1107-3756
Abstract
. Platelet-rich plasma (PRP) is derived from fresh whole blood, which contains a high concentration of platelets. Recently, PRP has been used for skin wound healing and rejuvenation. However, the molecular mechanisms underlying PRP-inducing wound healing processes are still largely unknown. The aim of this study is to evaluate the effect of PRP on the expression of G1 cell cycle regulatory proteins, type I collagen, matrix metalloproteinase-1 (MMP-1), and MMP-2 in human skin fibroblasts (HSF). We performed a cell proliferation and a migration assay, immunoblotting, and a chloramphenicol acetyltransferase (CAT) assay in PRP-treated human skin fibroblasts. PRP treatment induced increased rates of cell proliferation and cell migration. Expression of cyclin A protein was increased by a low concentration (0.5%) of PRP-treated HSF. In addition, expression of Rb, cyclin E, and cyclin-dependent kinase 4 proteins was increased by a high concentration (5%) of PRP-treated HSF. High concentration of PRP induced an up-regulation of type I collagen, MMP-1, and MMP-2 expression in HSF. Taken together, PRP treatment induced an increase in expression of G1 cell cycle regulators, type I collagen and MMP-1, thereby accelerating the wound healing process.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35962
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Keimyung Author(s)
조재위; 김성애; 이규석
Full Text
https://www.spandidos-publications.com/ijmm/29/1/32
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