계명대학교 의학도서관 Repository

Increased Expression of ATP-sensitive K+ Channels Improves the Right Ventricular Tolerance to Hypoxia in Rabbit Hearts

Metadata Downloads
Affiliated Author(s)
송대규
Alternative Author(s)
Song, Dae Kyu
Journal Title
Korean J of Physiology & Pharmacology
ISSN
1226-4512
Issued Date
2011
Keyword
ATP-sensitive K+ channelsKir6.2Right ventricleHypoxia
Abstract
ATP-sensitive K+ channels (KATP) are major component of preventing ischemia-reperfusion injury.
However, there is little information regarding to the expressional difference of KATP and its function
between left and right ventricles. In this study, we measured the lactate dehydrogenase release of
rabbit heart slices in vitro and determined the difference of the KATP expression at the both ventricles
by measuring the level of KATP-forming Kir6.2 (OcKir6.2) mRNA using in situ hybridization. The hearts
were preconditioned with 15 min hypoxia and reoxygenated for 15 min before a hypoxic period of 60
min, followed by reoxygenation for 180 min. With hypoxic preconditioning (100% N2) with 15 min,
left ventricles (LV) showed higher release of LDH comparing with right ventricles (RV). Adding KATP
blocker glibenclamide (10μM) prior to a hypoxic period of 60 min, hypoxic preconditioning effect of
RV was more abolished than LV. With in situ hybridization, the optical density of OcKir6.2 was higher
in RV. Therefore, we suggest that different KATP expression between LV and RV is responsible for
the different response to hypoxia and hypoxic preconditioning of rabbit hearts.
Department
Dept. of Physiology (생리학)
Publisher
School of Medicine
Citation
Hyoung Kyu Kim et al. (2011). Increased Expression of ATP-sensitive K+ Channels Improves the Right Ventricular Tolerance to Hypoxia in Rabbit Hearts. Korean J of Physiology & Pharmacology, 15(4), 189–194. doi: 10.4196/kjpp.2011.15.4.189
Type
Article
ISSN
1226-4512
DOI
10.4196/kjpp.2011.15.4.189
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36110
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.