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The effect of parental imprinting on the INS-IGF2 locus of korean type 1 diabetic patients

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Affiliated Author(s)
김흥식이인규장성익
Alternative Author(s)
Kim, Heung SikLee, In KyuChang, Sung Ik
Journal Title
Korean Journal of Internal Medicine
ISSN
1226-3303
Issued Date
2001
Keyword
Genomic imprintingDiabetes MellitusINSIGF2Korea
Abstract
Background : Insulin-dependent diabetes mellitus (IDDM) is caused by the autoimmune destruction of pancreatic β-cells. Susceptibility to IDDM appears to depend on more than one genetic locus. Evidence of a genetic linkage for IDDM2 was found in male meioses from French and North American populations. It is linked to maternal imprinting (i.e. monoalleleic expression of the insulin gene) that is considered the most likely cause of these gender-related differences. IGF2 is expressed only in the paternal allele and, therefore, is considered a candidate gene for IDDM2 transmission because of its important autocrine/paracrine effects on the thymus, lymphocytes and pancreas. Nevertheless, it remains controversial whether the parental origin of IDDM2 influences IDDM susceptibility. Methods : Using PCR and semi-quantitative RT-PCR, we analyzed the INS/PstI+1127 and IGF2/ApaI polymorphisms and RNA expression level between PstI (+/−) and PstI (+/+) to determine genotype and allele-specific expression of the INS and IGF2 genes. Results : INS/PstI (+/+) and IGF2/ApaI (+/−) were observed in 36 (97.3%) of 37 IDDM patients and in 29 (72.5%) of 40 IDDM patients, respectively. The presence of both IGF2 alleles in RNA was observed in 21 (91.6%) of 24 IDDM patients. Our results show a 3-fold increase in RNA expression from PstI (+/−) allele over PstI (+/+) allele. Conclusion : Our conclusion does not entirely exclude IGF2 as the gene involved in IDDM2, even though the parental effect of IDDM2 transmission is not related to IGF2 maternal imprinting. The INS genotype appeared mostly in the PstI (+/+) homozygote and, therefore, we could not explain the INS imprinting pattern in Korean type 1 diabetic patients. Genetic differences between populations may account for the discrepancy between Korean type I diabetic patients and American or French type I diabetic patients.
Department
Dept. of Pediatrics (소아청소년학)
Dept. of Internal Medicine (내과학)
Dept. of Anatomy (해부학)
Publisher
School of Medicine
Citation
Heung Sik Kim et al. (2001). The effect of parental imprinting on the INS-IGF2 locus of korean type 1 diabetic patients. Korean Journal of Internal Medicine, 16(4), 223–229. doi: 10.3904/kjim.2001.16.4.223
Type
Article
ISSN
1226-3303
DOI
10.3904/kjim.2001.16.4.223
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36148
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pediatrics (소아청소년학)
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