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Epigallocatechin gallate reduces hypoxia-induced apoptosis in human hepatoma cells

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Affiliated Author(s)
신동훈정우진황재석배재훈
Alternative Author(s)
Shin, Dong HoonChung, Woo JinHwang, Jae SeokBae, Jae Hoon
Journal Title
Life Science
ISSN
0024-3205
Issued Date
2006
Keyword
ApoptosisEpigallocatechin gallateHepG2 cellsHypoxia
Abstract
Cell detachment from extracellular matrix is closely related to induction of apoptosis. Epigallocatechin gallate (EGCG) has been shown to have
antioxidant effect and to protect hypoxia-induced damage. We investigated whether EGCG reduced hypoxia-induced apoptosis and cell
detachment in HepG2 cells. EGCG prevented cell death by hypoxia (0.5% O2) in a dose-dependent manner (hypoxic cell viability, 54.67%). RT-PCR and caspase3 activity assay showed that the hypoxia-induced cell death was caused by apoptosis increasing mRNA level of BAX, CASP3, and caspase3 activity. EGCG reduced increase of these mRNA and caspase3 activity. Western blot analysis and immunocytochemistry showed that EGCG increased cell adhesion proteins including E-cadherin (CDH1), tumor-associated calcium signal transducer 1 (TACSTD1), and protein tyrosine kinase 2 (PTK2) decreased by hypoxia. Hypoxia-induced apoptosis in HepG2 cells, and EGCG contributed to the HepG2 cell survival by attenuating the apoptosis.
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