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주기성 호중구 감소증 환아에서 G-CSF 및 GM-CSF의 혈중 농도와 조혈성장인자에 대한 CFU-GM 집락형성능의 변화

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Affiliated Author(s)
김흥식전동석
Alternative Author(s)
Kim, Heung SikJeon, Dong Seok
Journal Title
소아과
ISSN
0560-3560
Issued Date
2001
Keyword
Cyclic neutropeniaG-CSFGM-CSFSCFCFU-GMCD34
Abstract
Purpose : Serum levels of G-CSF and GM-CSF were measured and CFU-GM assay using GCSF,
GM-CSF and SCF was conducted to evaluate the influence of hematopoietic growth factor
on the precursor cells of cyclic neutropenia.
Methods : A 7-year-old male with cyclic neutropenia was studied. Marrow mononuclear cells
were isolated at neutrophil nadir and recovery and cultured in methylcellulose media with or without
G-CSF, GM- CSF and SCF. CD34 positive cells were evaluated using flow cytometry. Serum
levels of G-CSF and GM-CSF were measured by ELISA.
Results : The Numbers of CFU-GM without growth factors were 50 at neutrophil nadir and 33
at the recovery phase in the patient and show increased colony forming capacity. CD34 positive
cells were 9.32% at nadir and 14.17% at recovery. Increasement of CFU-GM with G-CSF at nadir
and recovery were 46% and 118% and those with GM-CSF were 70% and 78% respectively,
compared with 54.4% and 78.2% in control groups. In contrast, the presence of SCF did not enhance
CFU-GM number in the patient, but in the control group, increasement with SCF was 28.9
%. There an was inverse relationship between serum G-CSF levels and peripheral neutrophil count
whereas those of GM-CSF were constant.
Conclusion : Serum G-CSF level showed inverse relationship with neutrophil counts. The response
of progenitor cells to G-CSF and GM-CSF was not impaired. The presence of SCF did not enhance
CFU-GM number in the patient. This result suggests that the abnormality in hematopoiesis
in cyclic neutropenia may involve more immature progenitor cells responsive to SCF. (J Korean
Pediatr Soc 2001;44:1168-1175)
Alternative Title
Serum Level of G-CSF and GM-CSF and CFU-GM
Response to Hematopoietic Growth Factors in a
Patient with Cyclic Neutropenia
Department
Dept. of Pediatrics (소아청소년학)
Dept. of Laboratory Medicine (진단검사의학)
Publisher
School of Medicine
Citation
이연경 et al. (2001). 주기성 호중구 감소증 환아에서 G-CSF 및
GM-CSF의 혈중 농도와 조혈성장인자에 대한
CFU-GM 집락형성능의 변화. 소아과, 44(10), 1168–1175.
Type
Article
ISSN
0560-3560
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36679
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
1. School of Medicine (의과대학) > Dept. of Pediatrics (소아청소년학)
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