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백서의 혈관 평활근 세포 증식에 대한 E2F Decoy Oligodeoxynucleotides의 효과

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Affiliated Author(s)
김혜순한성욱이인규김기식박남희최세영
Alternative Author(s)
Kim, Hye SoonHan, Seong WookLee, In KyuKim, Kee SikPark, Nam HeeChoi, Sae Young
Journal Title
한국지질동맥경화학회
ISSN
1226-4393
Issued Date
2003
Keyword
E2F decoyNeointimal formationVSMC proliferationBalloon injury
Abstract
Background : Excessive proliferation of vascular smooth muscle cells (VSMCs) and neointimal formation are
critical steps in the pathogenesis of restenosis after percutaneous transluminal angioplasty (PTCA). The transcription
factor, E2F, plays a critical role in transactivation of several genes involved in cell cycle regulation. In the present
study, we investigated the hypothesis that transfection of cis-element double-stranded decoy oligodeoxynucleotides
(ODNs) corresponding to E2F binding sites inhibits the proliferation of VSMCs and neointimal hyperplasia.
Methods : We evaluated gene expression and proliferation in rat VSMCs under high D-glucose and serum, and
after transfection of E2F decoy ODNs in vitro. And we also evaluated neointimal formation in vivo model of rat
carotid injury.
Results : Transfection with E2F decoy ODNs inhibited VSMCs proliferation (p<0.05). Transfection of E2F decoy
ODNs attenuated DNA binding activity (p<0.001) and promoter activity of E2F induced by high glucose and serum
(P<0.01) E2F decoy ODNs also attenuated glucose- and serum-induced expression of cyclin A and PCNA genes
(p<0.01). Administration of E2F decoy ODNs in vivo using the hemagglutinating virus of Japan (HVJ)-liposome
method virtually abolished neointimal formation after ballon injury to the rat carotid artery (P<0.01).
Conclusion : Conclusions: This study shows that transfection of E2F decoy ODNs may decrease cell cycle
regulatory gene expression, cell proliferation and vascular lesion formation in vitro and in vivo. Our results provide
a novel potential therapeutic strategy for the prevention of restenosis after angioplasty.
Alternative Title
Effect of the E2F decoy Oligodeoxynucleotides in Rat Vascular Smooth Muscle Cell Proliferation
Department
Dept. of Internal Medicine (내과학)
Dept. of Thoracic & Cardiovascular Surgery (흉부외과학)
Publisher
School of Medicine
Citation
김미정 et al. (2003). 백서의 혈관 평활근 세포 증식에 대한 E2F Decoy Oligodeoxynucleotides의 효과. 한국지질동맥경화학회, 13(2), 396–406.
Type
Article
ISSN
1226-4393
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/37163
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Thoracic & Cardiovascular Surgery (흉부외과학)
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