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Switching-on of serotonergic calcium signaling in activated hepatic stellate cells

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Affiliated Author(s)
김나현
Alternative Author(s)
Kim, Na Hyun
Journal Title
World Journal of Gastroenterology
ISSN
1007-9327
Issued Date
2011
Abstract
AIM: To investigate serotonergic Ca2+ signaling and the
expression of 5-hydroxytryptamine (5-HT) receptors,
as well as Ca2+ transporting proteins, in hepatic stellate
cells (HSCs).
METHODS: The intracellular Ca2+ concentration ([Ca2+]i)
of isolated rat HSCs was measured with a fluorescence
microscopic imaging system. Quantitative PCR was performed
to determine the transcriptional levels of 5-HT
receptors and endoplasmic reticulum (ER) proteins involved
in Ca2+ storage and release in cultured rat HSCs.
RESULTS: Distinct from quiescent cells, activated HSCs
exhibited [Ca2+]i transients following treatment with
5-HT, which was abolished by U-73122, a phospholipase
C inhibitor. Upregulation of 5-HT2A and 5-HT2B receptors,
but not 5-HT3, was prominent during trans-differentiation
of HSCs. Pretreatment with ritanserin, a 5-HT2
antagonist, inhibited [Ca2+]i changes upon application
of 5-HT. Expression of type 1 inositol-5’-triphosphate
receptor and type 2 sarcoplasmic/endoplasmic reticulum
Ca2+ ATPase were also increased during activation of
HSCs and serve as the major isotypes for ER Ca2+ storage
and release in activated HSCs. Ca2+ binding chaperone
proteins of the ER, including calreticulin, calnexin
and calsequestrin, were up-regulated following activation
of HSCs.
CONCLUSION: The appearance of 5-HT-induced [Ca2+]i
response accompanied by upregulation of metabotropic
5-HT2 receptors and Ca2+ transporting/chaperone ER
proteins may participate in the activating process of
HSCs.
Department
Dept. of Nursing (간호학)
Publisher
College of Nursing
Citation
Kyu-Sang Park et al. (2011). Switching-on of serotonergic calcium signaling in activated hepatic stellate cells. World Journal of Gastroenterology, 17(2), 164–173. doi: 10.3748/wjg.v17.i2.164
Type
Article
ISSN
1007-9327
DOI
10.3748/wjg.v17.i2.164
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/37234
Appears in Collections:
2. College of Nursing (간호대학) > Dept. of Nursing (간호학)
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