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1p36.22 region containing PGD gene is frequently gained in human cervical cancer

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Affiliated Author(s)
조치흠
Alternative Author(s)
Cho, Chi Heum
Journal Title
Journal of Obstetrics and Gynaecology Research
ISSN
1341-8076
Issued Date
2014
Abstract
Aim: To identify commonly occurring DNA copy number alterations in Korean cervical cancers.
Methods: DNA copy number alteration was screened by whole-genome array comparative genomic hybrid-
ization (CGH) analysis. For the array CGH discovery, genomic DNA from five cervical cancers and 10 normal
cervical tissues were examined. For the independent validation of the most significant chromosomal alteration
(1p36.22, PGD gene), 40 formalin-fixed paraffin-embedded cervical tissue samples were collected; 10 of them
were used for quantitative polymerase chain reaction and the other 30 samples were used for immunohis-
tochemical analysis. Chromosomal segments differently distributed between cancers and normal controls
were determined to be recurrently altered regions (RAR).
Results: A total of 13 RAR (11 RAR losses and two RAR gains) were defined in this study. Of the 13 cervical
cancer-specific RAR, RAR gain in the 1p36.22 locus where the PGD gene is located was the most commonly
detected in cancers (P = 0.004). In the quantitative polymerase chain reaction replication, copy number gain of
the PGD gene was consistently identified in cervical cancers but not in the normal tissues (P = 0.02). In
immunohistochemical analysis, PGD expression was significantly higher in cervical cancers than normal
tissues (P = 0.02).
Conclusion: Our results will be helpful to understand cervical carcinogenesis, and the PGD gene can be a
useful biomarker of cervical cancer.
Key words: array comparative genomic hybridization, cervical cancer, copy number alteration, PGD, recur-
rently altered region.
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Publisher
School of Medicine
Citation
Maria Lee et al. (2014). 1p36.22 region containing PGD gene is frequently gained in human cervical cancer. Journal of Obstetrics and Gynaecology Research, 40(2), 545–553. doi: 10.1111/jog.12193
Type
Article
ISSN
1341-8076
DOI
10.1111/jog.12193
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/37265
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
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