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MAGE-3 암특이항원을 지령하는 DNA백신 개발을 위한 MAGE-3 발현성 Plasmid의 구축

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Affiliated Author(s)
박종욱백원기서성일서민호
Alternative Author(s)
Park, Jong WookBaek, Won KiSuh, Seong IlSuh, Min Ho
Journal Title
대한암학회지
ISSN
0496-6872
Issued Date
2000
Abstract
Purpose: The spectrum of melanoma antigen gene (MAGE)-expressing tumor is very wide and the gene of MAGE express antigens that are targets for specific recognition by cytotoxic T lymphocytes derived from tumor-bearing patients. All of these characteristics represent MAGE as tumor vaccine can be useful for cancer prevention or treatment. Here, we detected MAGE-3 gene expression in cancer cell lines and evaluated recombinant MAGE-3 protein producibility of MAGE plasmid to develope MAGE DNA vaccine. Materials and Methods: MAGE-3 gene expression of cancer cell lines was evaluated by reverse transcription-polymerase chanin reaction (RT-PCR). Two kinds of MAGE-3 expressing plasmids were constructed and their MAGE-3 protein producibility was evaluated by immunohistochemistry and immunoblotting using monoclonal anti-MAGE-3 antibody.
Results: Among 13 cell lines, SNU484, AMC-HN-3, AMC-HN-4, AMC-HN-7, HeLa, NCI H1703 and HT29 expressed MAGE-3 mRNA. In order to make MAGE plasmid, cDNA that showed 100% DNA homology with MAGE-3 gene was cloned into pcDNA 3 plasmid and pSecTag plasmid. Intracytoplasmic and secretory recombinant MAGE-3 was produced by MAGE-3 containing pcDNA 3 plasmid and pSecTag plasmid, respectively. Conclusion: In this study, we showed high expression frequency of MAGE-3 in cancer cell line, and established two kinds of plasmid that produce recombinant MAGE-3 in cell lines. We expect these plasmids will be used in cancer treatment or MAGE-3 function study in future.
Alternative Title
Construction of MAGE-3 Expressing Plasmid for Development of DNA Vaccine Encoding MAGE-3 Cancer Antigen
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
Publisher
School of Medicine
Citation
박종욱 et al. (2000). MAGE-3 암특이항원을 지령하는 DNA백신 개발을 위한 MAGE-3 발현성 Plasmid의 구축. 대한암학회지, 32(1), 191–199.
Type
Article
ISSN
0496-6872
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38090
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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