한국인 위암 세포주에서 세포주기 조절 유전자의 발현

Other Titles
Expression of Cell Cycle Control Genes in Korean Gastric Cancer Cell Lines
Authors
임춘철김인호조재위백원기서성일서민호박종욱강중신손수상
Department
Dept. of Surgery (외과학); Dept. of Microbiology (미생물학); Dept. of Immunology (면역학)
Issue Date
2000
Citation
대한암학회지, Vol.32(2) : 279-287, 2000
ISSN
0496-6872
Abstract
Purpose: In order to elucidate the roles of the cell cycle control genes in the carcinogenesis of Korean gastric cancer, the expressions of cyclin A, D1,E, p16INK4a and Rb in Korean gastric cancer cells were investigated in this study. Materials and Methods: The expression patterns of major cell cycle control genes in Korean gastric cancer cells (SNU1, 5, 216,484, 601, 620,638, 668,710) were analysed by using western blot and reverse transcription-polymerase chain reaction (RT-PCR). Results: In most of the gastric cancer cells investigated, the overexpressions of cyclin A and hyperphosphorylated Rb, and markedly decreased expression of p16INK4a were observed. Over-expression of cyclin E was also observed in SNU1, 484,601 and 638 gastric cancer cells. Conclusion: Considering all these findings, the following model of the gastric carcinogenesis caused by the altered expressions of the cell cycle control genes can be postulated: over-expressions of cyclin A and E cause acceleration of Rb phosphorylation, inactivation of Rb functions and acceleration of Gl/S transition of the cells; decreased expression of pl6 8 causes inadequate inhibition of cyclin-CDK and further acceleration of Rb phosphorylation; consequently, Rb inactivation, G1 shortening, inappropriate cell division and decreased function of the maintenance of genomic stability occurs. All these abnormalities should contribute to the carcinogenesis of Korean gastric cancer cells.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/38109
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
Keimyung Author(s)
김인호; 강중신; 손수상; 백원기; 서성일; 서민호; 박종욱
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